Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,919,753	C→T	G332R (GGG→AGG)	gudX ←	glucarate dehydratase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,919,753	0	C	T	100.0%	31.5 / NA	14	G332R (GGG→AGG)	gudX	glucarate dehydratase‑related protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/8);  total (6/8)

TTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTACCCGGTGCCGCCGCGCCCACATGGGTAAACATCGCCAGAGAGATATCGAAATGGTTATTAGAATGGCAGCCCCAGGTCAGCCCCCAGTCGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAG  >  NC_000913/2919524‑2919996                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ttttGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTACCCGGTGCCGCCGCGCCCACATGGGTAAACATCGCCAGAGAGATATCGAAATGGTTATTAGAATGGCAGCCCCAGGTCAGCCTCCAGTCGTCGCAAAGCTgcg                                                                                                                                                                                                                                 <  1:17318/250‑1 (MQ=255)                                       ggtcgtaTCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTGTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTACCCGGTGCCGCCGCGCCCACATGGGTAAACATCGCCAGAGAGATATCGAAATGGTTATTAGAATGGCAGCCCCAGGTCAGCCTCCAGTCGTCGCAAAGCTgcgcn                                                                                                                                                                                                                               <  1:54780/208‑2 (MQ=255)                                                aGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGGCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTACCCGGTGCCGCCGCGCCCACATGGGTAAACATCGCCAGAGAGAGAGCGAAAGGGTTATTAGAATGGCAGCCCCAGGTCAGCCTCCAGTCGTTGCAAAGCTGCGCCACACTGGCTGCGCCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTg                                                                                                                                                                                 <  1:104166/251‑1 (MQ=255)                                                      ggCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGACCTACTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTACCCGGTGCCGCCGAGCCCACATGGGTAAACATCGCCAGAGAGATATCGAAATGGTTATTAGAATGGCAGCCCCAGGTCAGCCTCCAGTCGTCGCAAAGCTGCGCCACACGGACTACACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAatat                                                                                                                                                                           >  1:20725/1‑251 (MQ=255)                                                          aTTTTCCCGTTTTTAACCCCCCGCGGATTTTGGGTCAGGCGCCAACCGCCCCCCAGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTACCCGGTGCCGCCGCGCCCACATGTGTAAACATCGCCAGAGAGATATCGAAATGGTTATTAGAATGGCAGCCCCAGGTCAGCCTGCAGTCGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTan                                                                                                                                                                       <  1:156375/251‑2 (MQ=255)                                                                                                                tGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTACCCGGTGCCGCCACGCCCACATGGGTAAACATCGCCAGATAGATATCGAAATGGTTATTAGAATGGCAGCCCCAGGTCAGTCTCCAGTCGTCGCAAAGCTGCGGCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGc                                                                                                                                                             <  1:96999/207‑1 (MQ=255)                                                                                                                                   cGATAGCGGTAGGATTACCCGGTGCCGCCGCGCCCACATGGGTAAACATCGCCAGAGAGATATCGAAATGGTTATTAGAATGGCAGCCCCAGGGCAGCCTCCAGTCGTCGCAAAGGTGCGCCACACTGACTGCTCCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCAt                                                                                                                                            <  1:104110/205‑1 (MQ=255)                                                                                                                                                   aCCCGGTGCCGCCGCGCCCACATGGGGAAACATCGCCAGAGAGATATCGAAATGGTTATTAGAATGGCAGCCCCAGGTCAGCCTCCAGTCGTCGCAAAGCTGCGCCacac                                                                                                                                                                                                                           <  1:245425/110‑1 (MQ=255)                                                                                                                                                    cccGGTGCCGCAGCGCCCACATGGGTAAACATCGCCAGAAAGATATCGAAATGGTTATTAGAATGGCAGCCTCCGGACAGCCTCCAGTCGTCGAAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGAGGAATATCTACCGCCTTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGCTCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTc                                                                             >  1:133038/1‑251 (MQ=255)                                                                                                                                                                                                        ggTTATTAGAATGGAAGCCCAAGGTCAGCCTCCAGTCGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGtt                                                                                                                 >  1:70528/1‑163 (MQ=255)                                                                                                                                                                                                                gAATGGCAGCCCCAGGTCACCCTCCAGTCGTCGCAAAGCTGCGCCACACCGCCTCCACCGGGAAGCGTCCACACGTGCGCATCGGCAAGTGGAACATCTACCGc                                                                                                                                                                    >  1:101264/1‑104 (MQ=255)                                                                                                                                                                                                                        nccccAGGTCAGCCTCCAGTCGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTcgcg                                                                                                                                      >  1:31191/2‑126 (MQ=255)                                                                                                                                                                                                                              ggTCAGCCTCCAGTCGTCGCAAAGCTGCGCCACACGGACTGCACCGGACGGCGTCCAGCACTGCGGATCGGCCAGTGGAATATCTACCGCCTTGAGCATCACCGCATGAACCAttt                                                                                                                                          >  1:230027/1‑116 (MQ=255)                                                                                                                                                                                                                               gTCAGCCTCCAGTCGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAg  <  1:105073/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    TTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTACCCGGTGCCGCCGCGCCCACATGGGTAAACATCGCCAGAGAGATATCGAAATGGTTATTAGAATGGCAGCCCCAGGTCAGCCCCCAGTCGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAG  >  NC_000913/2919524‑2919996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑