Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	197,886	C→T	F447F (TTC→TTT)	rseP →	intramembrane zinc metalloprotease RseP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	197,886	0	C	T	100.0%	31.5 / NA	13	F447F (TTC→TTT)	rseP	intramembrane zinc metalloprotease RseP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/6);  total (7/6)

CCTGTTTCCGTTGCCCGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGC  >  NC_000913/197727‑198119                                                                                                                                                                |                                                                                                                                                                                                                                          ccTGTTTCCGTTGCCCGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTTTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAgcgc                                                                                                                                                   >  1:8437/1‑248 (MQ=255)          gTTGCCGGGACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTAACCGAGCGGGTTCAAGACTTTTGTTAGCGCATTGGCTCGATGCTGCTGGTGTTGTTAATGGGGCTTGCACTGTTCAATGATTTTGCTCGGTTATGAGAGTTAGTTAGGATGAACGCATAATAACGATTGCGATGAAAAAGTTGCTCATAgn                                                                                                                                                                        <  1:29532/218‑2 (MQ=255)               ccGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGTTCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTTTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCtn                                                                                                                                  <  1:154291/251‑2 (MQ=255)                                                cNATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTTTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGa                                                                                                   >  1:198699/1‑249 (MQ=255)                                                cGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTTTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGctgctg                                                                                                                                                              >  1:178350/1‑190 (MQ=255)                                                                       ncGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTTTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGaa                                                                                                                                                                                       >  1:49946/2‑142 (MQ=255)                                                                              ccGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTTTCTCGGTTATGAGCGTTAGCTAGGAAGAACGCATCATAAAGATGGCGATGAAAAAGt                                                                                                                                                                                  >  1:126230/1‑140 (MQ=255)                                                                                      gTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTTTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGtt                                                                             >  1:4150/1‑233 (MQ=255)                                                                                       ttCAAGACTTTTGTTATCGCATTGGCTCGATCCTGCTGGTGCCGTTAATGGGGCTTGCACTTTGCAATGATTTTTCTCGGTAATGAGAGGTAGTGAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCAGAGCGTCGCTGCTGTTTag                                                                                                                                                         <  1:130949/156‑1 (MQ=255)                                                                                              cTTTTGTTATCGTATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTTTCTCGGTTATGAGAGTTAGTGAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCGGCTGTTTAGCAGCGCCACCGGATTCGGTGCTGAAGGGTTc                                                                                                                          <  1:83468/180‑1 (MQ=255)                                                                                                               gCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTTTCTCGGTTATGAGATTTAGTTAGGAAGAACGCataat                                                                                                                                                                                                      <  2:17075/87‑1 (MQ=255)                                                                                                               gCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTTTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCataat                                                                                                                                                                                                      >  1:17075/1‑87 (MQ=255)                                                                                                                                               gCACTTTTCAATGATTTTTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCGTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTcgc  <  1:131367/251‑1 (MQ=255)                                                                                                                                                      tNNATGATTNNTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAgcgc                                                                                                                                                   <  2:8437/99‑1 (MQ=255)                                                                                                                                                                |                                                                                                                                                                                                                                          CCTGTTTCCGTTGCCCGTACTTGACGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGACCGGTATCCGAGCGGGTTCAAGACTTTTGTTATCGCATTGGCTCGATTCTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTATGAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGTGCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGTGCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGTAATACCATTCGC  >  NC_000913/197727‑198119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑