Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,887	Δ1 bp	coding (678/1221 nt)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,887	0	C	.	100.0%	30.1 / NA	9	coding (678/1221 nt)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (4/5);  total (4/5)

AAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACA  >  NC_000913/1667719‑1668049                                                                                                                                                                         |                                                                                                                                                                   aaGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCa                                                                                 <  1:97905/251‑1 (MQ=255)                                      cTGGCNNTGNNNTCNNGGCAGCCGTGATTCCCGCAATAANAGCGTTTCCCATACGGGTCGACCTGTGTGNGNCNTATTNNCACNAGACTCCTGCNGCCTGCGTGTAGCAGATGACCATCGGTACTGAAGCC‑GCCACCACGTTGNGNTCGCTCACAACCTGAATCAAATCGCGCGCCCNGCGTGAGGCACCAAACAAgg                                                                                                 >  2:185148/1‑198 (MQ=255)                                      cTGGCGATGGTTTCGATGCAGCCGTGATTCCCGCAATACCAGCGTTTCCCATACGGGTCGACCTTTGTGTGGCCTATTTCCACAAGACTACTGCTGCCTGCGTGTAGCAGAGGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCGGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAgg                                                                                                 <  1:185148/198‑1 (MQ=255)                                                tttCGNNGCNNNCGNNATTCCCGCAATAACAGCGTTTCCNATACGGGTCGACCTGTGTGTGGCCTATTTNCNCNAGACNNCTGNTGCCTGCGTGNAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAANCNCCTGAATc                                                                                                                                   >  2:191608/1‑154 (MQ=255)                                                tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGGCCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATc                                                                                                                                   <  1:191608/154‑1 (MQ=255)                                                                                cGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGATGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGc                      <  1:177725/231‑1 (MQ=255)                                                                                           cGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGc                      <  1:44273/220‑1 (MQ=255)                                                                                                   ncTGTGTGTGGCCTATTTCCACGAGACTCCTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACa  >  1:77579/2‑232 (MQ=255)                                                                                                                                              tAGCAGATGACCATCGGTAATGACGCC‑GCCCGCACGTTGTGCTCGATAACCACCAGAATCACATCGCGCGCCCCGCGTGAGGCACAAAACAAGGCCTCTACCATCGTCCATCCGCTGATATCa                                                                    >  1:106469/1‑123 (MQ=255)                                                                                                                                                                         |                                                                                                                                                                   AAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACA  >  NC_000913/1667719‑1668049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑