| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 580,115 | C→T | intergenic (+29/+136) | ybcW → / ← ylcI | uncharacterized protein YbcW/DUF3950 domain‑containing protein YlcI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 580,115 | 0 | C | T | 83.3% | 26.9 / 2.7 | 18 | intergenic (+29/+136) | ybcW/ylcI | uncharacterized protein YbcW/DUF3950 domain‑containing protein YlcI |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (7/8); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
AATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTG > NC_000913/579934‑580333 | aaTATGCTTAATAGCACTATTTCTATGAGTTACCCTGAGGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAAGTGAGGCAGCGGTGGTGAAGCACGATAATAAGATGAAGGATTATTCGCTGGTGGTTGACTGGTCACCATAATTGCTAATCATTCATACTATGGAGTCTGTGTCAGAGCCAACACGCATTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTAc < 1:34873/251‑1 (MQ=255) atatcACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGATGCAGCGTTGGTGAAGCACGATAAGAATATGAAGGATTATTCCCTGTTGGTTGACTGATCACCATAACTGCTAATCATGCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGccn < 1:25173/247‑2 (MQ=255) gCACCATTTCTCTGAGTTCCCCTGATGTTGTAATTACATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCg > 1:75431/1‑251 (MQ=255) cTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAat > 1:6757/1‑250 (MQ=255) ggTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTCGTGAAGCACGATCATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGATAATCATTCAAACTATTTAGTCTGTGACAGCGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATg > 1:147762/1‑251 (MQ=255) ggAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGAGTATTCCCTGGTGGTTGACTGAGCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGt < 1:39487/136‑1 (MQ=255) aaGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCAttcttcn < 1:151826/251‑2 (MQ=255) gTTGGTGAAGCACGATAATAATATGAAGGATTATTCTCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGTCAGAGCCAACACGCAGTCTGTCACTATCAGGAAAGTGGTAAAACTGCGACTCAACTACTGCAATGCGCTCTTTAATAAGATTATTTTCCCTGTTTTCCTGTTCTTAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTaa < 1:129299/236‑1 (MQ=255) gTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTAGACTGATCACCATACCTGCTAATCATTCCAACTATTTAGTCTGTGAAAGAGCCAACACGCAGTCTGTCACTGTCAGGAACGTGGTAAAACTGCAACTCAATTACTGCAATGc > 1:233294/1‑154 (MQ=255) gAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGc < 1:120119/133‑1 (MQ=255) aaGCACGATAATAATATGAAGTATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCATACTACTTAACCTGTGGCAGAGCCAACACGCAGTCTGTTACTGTCATGAAAGTGGTAAAACTGCAACTCAATTACTTCGAGGCCCTCGTAATGAAGTGAATTTACAATATCGGCCTTGTCGGAGTGGAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTc < 1:237344/251‑1 (MQ=255) cACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATTCCCTCGTAATTAAGTGAAGTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGa < 1:117828/251‑1 (MQ=255) ataataataTGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGGCACTGTCATGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGaa < 1:98406/161‑1 (MQ=255) ggtTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTtcatn < 1:69336/186‑2 (MQ=255) tGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGAAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTg > 1:114163/1‑250 (MQ=255) gATCACCATCACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCCGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAATGACCCAGGCTg > 1:71069/1‑249 (MQ=255) cACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACaa > 1:112985/1‑129 (MQ=255) aCCATAACNNCTNNTNANTCAGANTATTTAGTCTGAGACAGAGCCAACACGCAGTctgtcactgtc > 2:172253/1‑66 (MQ=255) | AATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTG > NC_000913/579934‑580333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |