Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,250,599	G→A	R13H (CGC→CAC)	yqjF →	DoxX family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,250,599	0	G	A	100.0%	22.9 / NA	10	R13H (CGC→CAC)	yqjF	DoxX family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

AGCAGCAACAGCTTCGCGGTTAACTTCCCTCTGGCCGGAGCCATTCCGGCCTTATCCCTCAAATTTTTTGAAGATTTTTGACAGTTTTCCTTGCTAACAATCATCATTCACCACGTTTATGATTCTCTCCATCGACAGCAACGACGCTAATACCGCGCCATTGCACAAAAAAACAATCAGCAGCCTGAGTGGCGCAGTGGAGAGTATGATGAAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGTTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTT  >  NC_000913/3250354‑3250814                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                        agcagcAACAGCTTCGCGGTTAACTTCCCTCTGGCCGGAGCCATTCCGGCCTTATCCCTCAAATTTTTTGAAGATTTTTGACAGTTTTCCTTGCTAACAATCATCATTCACCACGTTTATGATTCTCTCCATCGACAGCAACGACGCTAATACCGCGCCATTGCACAAAAAAACAATCAGCAGCCTGAGTGGCGCAGTGGAGAGTATGATGAAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCACAttt                                                                                                                                                                                                                    >  1:16468/1‑251 (MQ=255)                ncgGTTAACTTCCCTCTGGCCGGAGCCATTCCGGACTTATCCCTCAAATTTTTTGAAGATTTTTGACAGTTTTCCTTGCTAACAATCATCATTCACCACGTTTATGATTCTCTCCATCGACAGCAACGACGCTAATACCGCGCCCTTGCACAAAAAAACAATCAGCAGCCTGAGGGGCGCAGTGGAGAGTATGATGAAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCACATTTTAATGCCGATTCTGt                                                                                                                                                                                                     >  1:50182/2‑251 (MQ=255)                                                      aTCCCTCAAATTTTTTGAAGATTTTTGACAGTTTTCCTTGCTAACAATCATCATTCACCACGTTTATGATTCTCTCCATCGACAGCAACGACGCTAATACCGCTCCATTGCACAAAAAAACAATCAGCAGCCTGAGTGGCGCAGTGGAGAGTATGATGAAAAAATTATAAGATGTTGGTGTACTGGTAGCGCACAGTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTAcgc                                                                                                                                                                <  1:228270/250‑1 (MQ=255)                                                          cTCAAATTTTTTGAAGATTTTTGACAGTTTTCCTTGCTAACAATCATCATTCACCACGTTTATGATTCTCTCCATCGACAGCAACGACGATAATACCGCGCCATTGCACAAAAAAACAATCAGCAGCCTGAGTGGCGCAGTGGAGAGTATGATGAAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCACATTTTAATGCCGATTCTGTTTATTAcc                                                                                                                                                                                             >  1:205635/1‑217 (MQ=255)                                                            cAAATTTTTTGAAGATTTTTGACAGTTTTCCTTGCTAACAATCATCATTCACCACGTTTATGATTCTCTCCATCGACAGCAACGACGCTAATACCGCGCCATTGCACAAAAAAACAATCAGCAGCCTGAGTGGCGCAGTGGAGAGTATGATGAAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCACATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTAcc                                                                                                                                                         >  1:20271/1‑251 (MQ=255)                                                            cAAATTTTTTGAAGATTTTTGACAGTTTTCCTTGCTAACAATCATCATTCACCACGTTTATGATTCTCTCCATCGACAGCAACGACGCTAATACCGCGCCATTGCACAAAAAAACAATCAGCAGCCTGAGTGGCGCAGTGGAGAGTATGATGAAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCACATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTAc                                                                                                                                                          <  1:10527/250‑1 (MQ=255)                                                                                                                                                     aaTACCGCGCCATTGCACAAAAAAACAATCAGCAGCCTGAGTGGCGCAGTGGAGAGTATGATGAAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCACATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGTCGTCCCGGGTTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGtt                                                                <  1:102538/251‑1 (MQ=255)                                                                                                                                                             gcCATTGCACAAAAAAACAAGCAGCAGCCTGAGTGGCGCAGTGGAGAGTATTATGAAAAAATTAGAATATGTTGGGTTACGGGTAGCGCACAGTTTAATGTCGATTCTGTTGATTACTGCTGGCTGGCGAAAAATTACTGGCTACGCGTGTACCCAACAATATATGGAAGCAATggg                                                                                                                                  <  1:170000/177‑1 (MQ=255)                                                                                                                                                                                    gcagcCTGAGTGGCGCAGTGGAGAGTATGATGAAAAACTTAGAAGATGTCGGTGTACTGGTCGCGCACATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGCAATATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTACCGGGTTTTATGCTGCCCCTGGTGCTTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTg                                                      >  1:193437/1‑230 (MQ=255)                                                                                                                                                                                                gcgcAGTGGAGAGTATGATGAAAAAATTAGAAGATGTTGGCGTACTGGTAGCGCACATTTTCCTGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACACTATATGGACGCAATGGGCGTCCCGGGTTTTAt                                                                                                                   >  1:121103/1‑157 (MQ=255)                                                                                                                                                                                                                                                  gcgcacATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGTTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCAtttt  <  1:17339/220‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                        AGCAGCAACAGCTTCGCGGTTAACTTCCCTCTGGCCGGAGCCATTCCGGCCTTATCCCTCAAATTTTTTGAAGATTTTTGACAGTTTTCCTTGCTAACAATCATCATTCACCACGTTTATGATTCTCTCCATCGACAGCAACGACGCTAATACCGCGCCATTGCACAAAAAAACAATCAGCAGCCTGAGTGGCGCAGTGGAGAGTATGATGAAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGTTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTT  >  NC_000913/3250354‑3250814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑