Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	737,311	G→A	intergenic (+612/‑781)	ybfQ → / → ybfD	inactive transposase YbfQ/H repeat‑associated putative transposase YbfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	737,311	0	G	A	100.0%	22.9 / NA	11	intergenic (+612/‑781)	ybfQ/ybfD	inactive transposase YbfQ/H repeat‑associated putative transposase YbfD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/3);  total (8/3)

CTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCTGTAAAAGGAAACCAGGGGCGGCTTAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATGAGTG  >  NC_000913/737100‑737503                                                                                                                                                                                                                    |                                                                                                                                                                                                 cTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATAGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGACCGCTCCATGTCATAAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGACAAGAAATCCAATGAGATCACAGCTATCACTGAACTTCTTAACATGCTGAATATTAAAGGAAAAATCATCACAACTGATGCGATGGGtt                                                                                                                                                           >  1:8156/1‑251 (MQ=14)                                                              cTCCGGCACTCTTATTACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGAATATTAAAGGAAAAATCATCCCAACTGATGCGATGGGTTGCCAg                                                                                                                                                      <  1:6211/195‑1 (MQ=255)                                                                ncGGCACTCTTATGACAAGAGTCGCAGCAGGGGCGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGAATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGc                                                                                                                                                         >  1:76286/2‑190 (MQ=255)                                                                                    gTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGAATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCTGTAAAAGGAAACCAGGGGCGGCTtaat                                                                        >  1:201024/1‑251 (MQ=255)                                                                                                    gATTCATGTCAGTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATTATATCACAGCTATCCCTGAACTTCTTAACATGCTGAATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTGGCCAAAAAGATATTGCAGAGAAGATACAAAAGCAGGTAGGGGATTATTTAGTCGCTGTAAAAGGAAACCAGGGGCGGCTTAATAAAGcc                                                                  <  1:130414/241‑1 (MQ=11)                                                                                                                    gCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATATGAAATCCAATGCGATCACAGCTATCCCTGAACTTCTTAACATGCTGAATATTAAAGGAAAAATCATCACAACTGATGCGATGGGGTGCCAGAAAGa                                                                                                                                                 >  1:191527/1‑146 (MQ=14)                                                                                                                                                       gATCAAGACGGATAAGAAATACAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGAATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCTGTAAAAGGAAACCAGGGGCGGCTTAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCagag                        >  1:205746/1‑232 (MQ=255)                                                                                                                                                          naaGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGAATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCTGTAAAAGGAAACCAGGGGCGGCTTAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATGAGTg  >  1:48783/2‑251 (MQ=255)                                                                                                                                                               cGGATCAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGAATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGNGAtnnnnnat                                                                                                        >  2:154886/1‑144 (MQ=255)                                                                                                                                                               cGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTGCTTAACATGCTGAATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCTGTAAAAGGAAACCAgggn                                                                                 <  1:154886/167‑2 (MQ=255)                                                                                                                                                                                           tATCCCTGAAATTCTTAACATGCTGANTATTAANNGANAAATCATCACACCTGATGCNATNNNTNNNCNGAAAGNTANNGCAGAGAAGNTACAAAAACAGGGAGGTGATTATTTATTCGCTGTAAAAGGAAACCAGGGGCGGNTTTATAACANNTTTGAGGGAAAATTTTCGCTGnaaannannanaaanacn                           >  2:58704/1‑178 (MQ=11)                                                                                                                                                                                                                    |                                                                                                                                                                                                 CTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCTGTAAAAGGAAACCAGGGGCGGCTTAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGAGCATGACAGTTACGCAATGAGTG  >  NC_000913/737100‑737503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑