Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,917,717	G→A	G70S (GGC→AGC)	letB →	lipophilic envelope spanning tunnel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,917,717	0	G	A	100.0%	11.4 / NA	7	G70S (GGC→AGC)	letB	lipophilic envelope spanning tunnel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/1);  total (6/1)

AGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCTTTCTGGCTGCTGCCGTTCATCGCGCTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTT  >  NC_000913/1917488‑1917944                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    aGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCGTTCTGGCTGCTGCTATTCAGCGCGCTAATGATTGCCAGTTGGCTGATTTGTGACAGTTATCAGGACCGGGGTAATACCGTCATCATCGACTTTATGTCGGCGGATGGTATGGTTCCAGGCCGTACGCCTGTTCGTTATCAGAGCGTTGAAGTCGGAACAGTGc                                                                                                                                                                                                                <  1:249661/251‑1 (MQ=255)                    nacaTGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAACATAAACGCCGTATCTCACCTTTCTGGCTGCTGCCGTTCATCGCGCTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGAGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGcg                                                                                                                                                                                              >  1:25429/2‑250 (MQ=255)                                                             gATTAAAAATAAACGCCGTATCTCACCTTTCTGGCTGCTGCCGTTCATCGCGCTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGAGCGTTGAAGTCGGAACAGTGCAGGATATCAGcc                                                                                                                                                                                                    >  1:122881/1‑203 (MQ=255)                                                                               tATCTCACCTTTCTGGCTGCTGCCGTTCATCGCGCTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGAACCGGGGTAATACCGTCACCATAGACTTTATGTCGGCGGATGGTATTGTTCCAGGACGTACGCCTGTTCGTTATCAGAGCGTTGAAGTCg                                                                                                                                                                                                                         >  1:120298/1‑164 (MQ=255)                                                                                                                                            gACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGAGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCt                                                                     >  1:195637/1‑251 (MQ=255)                                                                                                                                                                                        gTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTAGTTATCAGAGCGTTGAAGTCGGAACAGGGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGCCAGCATCAAGTCCCATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAA‑GCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGGGGGAACTATATCGGCATGATGCCGggta                         >  1:165920/1‑250 (MQ=255)                                                                                                                                                                                                               gCCGTACGCCTGTCCGTTATCAGAGCGTTGACGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACttt  >  1:196019/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    AGCCGCTTACTTTGGGATGCACATGAGTCAGGAAACGCCCGCTTCGACGACTGAAGCGCAGATTAAAAATAAACGCCGTATCTCACCTTTCTGGCTGCTGCCGTTCATCGCGCTAATGATTGCCAGTTGGCTGATTTGGGACAGTTATCAGGACCGGGGTAATACCGTCACCATCGACTTTATGTCGGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTT  >  NC_000913/1917488‑1917944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑