Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,020,602	C→T	Y21Y (TAC→TAT)	ygfM →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,020,602	0	C	T	100.0%	22.0 / NA	10	Y21Y (TAC→TAT)	ygfM	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

ATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCG  >  NC_000913/3020422‑3020832                                                                                                                                                                                     |                                                                                                                                                                                                                                       aTTCCATTTATGCACAAGCCAGAGAAGCCGCTACCAGTATGTGGCGTCGGATGGATGCGCTGGCCTAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTCACTAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGAGCCGCTATCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCACTGCTACACCAACCCGTACCGATacaa                                                                                                                                                                     >  1:117898/1‑245 (MQ=255)                           gCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATACGGAAAGAAGATGATTGAACACTTTTTCAGGCACGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTATCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCtt                                                                                                                                                     >  1:179345/1‑238 (MQ=255)                                            ncGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTATCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTg                                                                                                                       >  1:48865/2‑251 (MQ=255)                                                              ggCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTATCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCGGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTgn                                                                                                      <  1:50429/250‑2 (MQ=255)                                                                               cccTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTATCAGGATGAAGCCGTCTGGTTCGCCGGGGGCNGCANNNNNa                                                                                                                                                                                                >  2:152128/1‑143 (MQ=255)                                                                                      cccGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTATCAGGATGAAGCCGTCTGTTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGc                                                                                                                                                              <  1:238976/170‑1 (MQ=255)                                                                                              gAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTATCAGGATGAAGCCGTCTgn                                                                                                                                                                                                                      <  1:204353/106‑2 (MQ=255)                                                                                                                              aaCAATTTTTCAGGACCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTATCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCgn                                     <  1:152128/251‑2 (MQ=255)                                                                                                                                                                   ggAACTGAAGCGCCGCTATCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACGCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGTTTGACTGGTATAACGGTGCACGGCGGATTGGCGCAATGTCTCGCTGGCAGCCACTGCGTTAGGCGCGATGTAGGCGTGCAGCGTGGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCg  <  1:175497/249‑1 (MQ=255)                                                                                                                                                                   ggAACTGAAGCGCCGCTATCAGGATGAAGCCGTCGGGTGCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTg                                                                                                                           <  1:205993/128‑1 (MQ=255)                                                                                                                                                                                     |                                                                                                                                                                                                                                       ATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCG  >  NC_000913/3020422‑3020832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑