Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,849,125	C→T	L97L (CTG→TTG)	sppA →	protease IV, a signal peptide peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,849,125	0	C	T	100.0%	31.3 / NA	12	L97L (CTG→TTG)	sppA	protease IV, a signal peptide peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

CAGGTCAGTGGTGGTGATTCGAAAGAAACGGCCAGTCGTGGCGCACTGCTGCTGGACATTTCTGGTGTGATCGTCGATAAACCCGACAGTTCTCAGCGGTTTAGTAAATTAAGCCGCCAGCTGCTTGGTGCCAGTTCCGATCGTCTGCAGGAAAACTCACTGTTTGATATCGTCAACACTATTCGCCAGGCGAAGGACGACCGCAATATCACCGGTATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATT  >  NC_000913/1848966‑1849343                                                                                                                                                                |                                                                                                                                                                                                                           cAGGTCAGTGGTGGTGATTCGAAAGAAACGGCCAGTCGTGGCGCACTGCTGCTGGACATTTCTGGTGTGCTCGTCGATCAACCCGACAGTTCTCAGCGGTTTAGTAAATTAAGCCGCCAGCTGCTTGGTGCCAGTTCCGATCGTCTGCAGGAAAACTCATTGTTTGATATCGTCCacac                                                                                                                                                                                                         >  1:117328/1‑179 (MQ=255)   ggTCAGTGGTGGTGATTCGAAAGAAACGGCCAGTCGTGGCGCACTGCTGCTTGACATTTCTGGTGTGATCAGCGATAAACCCGACAGTTCTCAGCGGTTTAGTAAATTAAGCCGCCAGCTGCTTGGTGCCAGTTCCGATCGTCTGCAGGAAAACTCATTGTTTGATATCGTCAACACTATTCGCCAGGCTAAGGACGACCGCAATATCACCGGTATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGAcn                                                                                                                               <  1:26643/251‑2 (MQ=255)                                         gcgcACTGCTGCTGGACATTTCTGGTGTGATCGTCGATAAACCCGACAGTTCTCAGCGGTTTAGTAAATTAAGCCGCCAGCTGCTTGGTGCCAGTTCCGATCGTCTACAGGCAAACTCATTGTTTGATATCGTCAACACTATTCGCCAGGCGAAGGACGACCGCAATATCACCGGTATTGTGATGGATCTTAAACACTTCGCAGGCGGCGAc                                                                                                                                >  1:96777/1‑212 (MQ=255)                                                gctgctgGACATTTCTGGTGTGATCGTCGATAAACCCGACAGTTCTCAGCGGTTTAGTAAAGTAAGCCGCCAGCTGCTTGGTGCCAGTTCCGATCGTCTGCAGGAAAACTCATTGTTTGATATCGTCAACACTATTCGCCAGGCGAATGACGACCGCAATATCACCGGTATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCg                                                                                    <  1:220489/249‑1 (MQ=255)                                                   gctgGAAATTTCTGGTGTGATCGTCGNTAAACCNNACNGTTCTCAGCGGTTTAGTAANTTNNNCNNNCNGCTGCNTGNNGCCAGTTCCNATCGTCTGCAGGAAAACTCATTGTTTGATATCGTCAACACTCTTCGCCAGGCGNAGGACGACCNNAATATCACCGGTATTGTGATGNATCNNANNANCTTNGcn                                                                                                                                         >  2:62782/1‑192 (MQ=255)                                                        aCATGTCTGGTTTGATCATCGATAAACCCGACAGTGCGCAGCGGTTTAGTAAATTAAGCCGCCAGCTGCTGGGGGCCAGTTCCGATCGTCTGCAGGAAAACTCATTGTTTGATATCGTCAACACTATGCGCCAGGCGAAGGACGACCGCAATATCACCTGTATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCATCTATGCACTACATCGGCAAAGCTCTGAAAGAGGTGCGTGACAGCggg                                                                          <  1:87744/251‑1 (MQ=255)                                                                      aTCGTCGATAAACCCGACAGTTCTCAGCGGTTTAGTAAATTAAGCCGCCAGCTGCTTGGTGCCAGTTCCGATCGTCTGCAGGAAAACTCATTGTTTGATATCGTCAACACTATTCGCCAGGCGAAGGACGACCGCAATATCACCGGTATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGc                                                            <  1:62782/251‑1 (MQ=255)                                                                                                    tttAGTAAATTAAGCCGCCAGCTGCTTGGTGCCAGTTCCGATCGTCTGCAGGAAAACTCATTGTTTGATATCGTCAACACTATTCGCCAGGCGAAGGACGACCGCAATATCACCGGTATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAACCCGGTTTATGCCGTTAGCGAGAACTACAGc                                         <  1:74904/240‑1 (MQ=255)                                                                                                        gTAAATTAAGCCGCCAGCTGCTTGGTGCCAGTTCCGATAGTCTGCAGGAAAACTCATTGTTTGATATCGTCAACACTATTCGCCAGGCGAAGGAAGACAGCAATATCACCGGTATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAAtattat                          >  1:229240/1‑251 (MQ=255)                                                                                                                    gccAGCTGCTTGGTGCCAGTTCCGATCGTCTGCAGGACAACTCATTGTTTGATATCGTCAACCCTATTCGAAAGGCGAAGGACGACCGCAATATCACCGGTATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAACGCTCTGACAGCGTTTCGTGACCGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTc              >  1:223301/1‑251 (MQ=255)                                                                                                                                gTGCCAGTTCCGATCGTCTGCAGGAAAACTCATTGTTTGATATCGTCAACACTCTTCGCCCGGCGACGGACGACCGCAATATCACCGGTATTGTGATGGATCTGAAAAACTTCGCAAGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGCGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGACGAGAACTACAGCCAGGCGCAATATTATCTCGCCAGTTTCGCCAATAAAAtt  >  1:229116/1‑251 (MQ=255)                                                                                                                                  gCCAGTGCCGAGCGGCTGCAGGAAAACTCATTGTTTTATATCGTCAACACTATTCGCCAGGCGAAGGACGACCGCAATATCAcn                                                                                                                                                                       <  1:23078/84‑2 (MQ=255)                                                                                                                                                                |                                                                                                                                                                                                                           CAGGTCAGTGGTGGTGATTCGAAAGAAACGGCCAGTCGTGGCGCACTGCTGCTGGACATTTCTGGTGTGATCGTCGATAAACCCGACAGTTCTCAGCGGTTTAGTAAATTAAGCCGCCAGCTGCTTGGTGCCAGTTCCGATCGTCTGCAGGAAAACTCACTGTTTGATATCGTCAACACTATTCGCCAGGCGAAGGACGACCGCAATATCACCGGTATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATT  >  NC_000913/1848966‑1849343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑