Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	846,229	C→T	A79T (GCA→ACA)	glnQ ←	L‑glutamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	846,229	0	C	T	100.0%	37.2 / NA	14	A79T (GCA→ACA)	glnQ	L‑glutamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/4);  total (10/4)

TTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGCCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGAC  >  NC_000913/845996‑846445                                                                                                                                                                                                                                          |                                                                                                                                                                                                                         gttCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTTTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGTTTCCTGGCGAATCAgg                                                                                                                                                                                                          <  1:87794/249‑1 (MQ=255)                naGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACAAGAAAGTTCGGAAGGGTAGTGATGTGAACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGTTTCCTGGCGAATCAGGCGCTCGTCAACTTTCg                                                                                                                                                                                          >  1:48214/2‑251 (MQ=255)                                                   caccaGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGTTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATc                                                                                                                                                             >  1:188527/1‑245 (MQ=255)                                                                                                        gCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCAAGCACGCGTAGCGGGCCAAACATCACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGATGGAACACCATACCTGTTTCCTGGCGAATCAGgcgc                                                                                                                                                                                                       >  1:166165/1‑150 (MQ=255)                                                                                                                       gCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGCCGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGTTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCg                                                                                                                                                                                     >  1:201944/1‑153 (MQ=255)                                                                                                                                                   cgcCACGCACGCGTAGCGGGCCAACCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGTNNCCTGGc                                                                                                                                                                                                                  >  2:22488/1‑96 (MQ=255)                                                                                                                                                   cgcCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGTTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGAGCGTTAACCTTCAGGCCATCGACAATCAg                                                                                                                                                           <  1:22488/151‑1 (MQ=255)                                                                                                                                                               gTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACCCCATACCTGTTTCCTGGCGAATCAGGCGATCGTCCACTTTCGGCTCGTTAACCTTCAGCCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGc                                                                                     >  1:118011/1‑209 (MQ=255)                                                                                                                                                                           aCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTTGAACACCATGCCTGTTTCCTGTCGAATCAGGCGCTCGTGAACTTTCGGATCGTTAACCTGCAGGCCATCGACAAGCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTac                                                                                                   <  1:42486/183‑1 (MQ=255)                                                                                                                                                                                          gcgcTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGTTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGCCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGcc                 >  1:201108/1‑250 (MQ=255)                                                                                                                                                                                                  aGATGNNGGNNNAGNNAAAACTGCTGGAACACCATACCTNTTTCCTGGCGAATCAGGCGCTCGTCAACTNTNGNATCGNNAACNTTCAGGCCATNGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGNANAAGGGTCGATTTaccg                                                                                                 >  2:188173/1‑162 (MQ=255)                                                                                                                                                                                                  aGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGTTTCCTGGCGAATCAGGCGCTCGTCAACTTGCGGATCGTTAACCTTCAGGCCATCGACAATCATATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTaccg                                                                                                 <  1:188173/162‑1 (MQ=255)                                                                                                                                                                                                        gtgAAGAGGTAAAACTGCTGGAACACCATACCTGTTTACTGGCGAATCCGACGATCGCCAACTTTCGGATCGTTAACCTTACGGCAATAAACACTCAGATGGCCGGAGGAGATTTCTTCCAGTTTGTTGATGAAGCGCAGCAGGGTCGATTTACCGGCACCGGCCGGCTCGATAATCACCACGACTTCGCCCTGCGCAATGTTCAAATCGATATTGCGCAGCCCCTGGGATGGGCCAAAGTGCTTGgagcc  >  1:230818/3‑249 (MQ=255)                                                                                                                                                                                                             gagGTAAAACTGCTGGAACACCATACCTGTTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATg                                                                                                                         >  1:74214/1‑127 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                         TTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGCCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGAC  >  NC_000913/845996‑846445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑