Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,734,583 G→A A151V (GCC→GTC)  yfiH ← purine nucleoside phosphorylase YfiH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,734,5830GA91.7% 21.9 / ‑6.0 12A151V (GCC→GTC) yfiHpurine nucleoside phosphorylase YfiH
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (6/5);  total (6/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

TCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCC‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATA  >  NC_000913/2734368‑2734788
                                                                                                                                                                                                                        |                                                                                                                                                                                                             
tctcATTTTCCGTATATGTACAACGTTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAGTGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCC‑ACTTCGAACGCTCGTGGACCAATTGCCGGCCCTAACCAGACGAGAATATTTTCCGGATTATCAGCAAAACAGGa                                                                                                                                                                             <  1:226957/250‑1 (MQ=255)
               atGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCC‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGACGAGAATATTTTCCGGATTATCAGCACAACAGGAAACCGTCTCTTcgatc                                                                                                                                                             >  1:11978/1‑247 (MQ=255)
                            nccgccgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGACAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCC‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGACGAGAATATTTTCCGGATTATCAg                                                                                                                                                                                       >  1:32925/2‑212 (MQ=255)
                                                                                                                cTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTACCCACCC‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGACGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCAAAGGGAGGCAGTCGGCAGtcatc                                                             >  1:168465/1‑250 (MQ=255)
                                                                                                                             gCACTTTCTTTAGCGTCTACTGCCAGAAACGCCTCGCGAACCTCCCCCCCC‑ACTTCGAACGCGCGTGTACCAATTGCCGGACCTAACCAGACGAGAATATTTTCCGGATTATCATCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGGCCACGCCAGCCAGCATGAGCGGCGGCGACTACCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAAcc                                                 <  1:99915/249‑1 (MQ=255)
                                                                                                                                                  gCCATAAACGCCCCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATAGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCGCGTCCAGCACGCCTGCGCACAGTCCGCGCTAGGCAGCATGGGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAgg                                                                            <  1:134092/202‑1 (MQ=255)
                                                                                                                                                        aaCGCCTCGCGAACCTCCCCCCCC‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGACGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCg                                                                                                                                                                        >  1:4619/1‑103 (MQ=255)
                                                                                                                                                                  gacccccccccccc‑ACTTCGAACGCGCGTGTACCAATTGCCGGCCCTAACCAGACGAGAATATTTTCCGGATTAGCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAACCAGCACAGGGATGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGtttn          <  1:23769/245‑2 (MQ=255)
                                                                                                                                                                     ccTCCCCCCCC‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGACGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCCTGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGc                                 >  1:105767/1‑225 (MQ=255)
                                                                                                                                                                           ncccc‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGACGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATa  >  1:67233/2‑250 (MQ=255)
                                                                                                                                                                                    ncGAACGNGCGTGGACCAATTGCCGGCNCTAACCAGANGAGAANNNTNTCCGNATGATCAg                                                                                                                                                                                       <  2:32925/60‑1 (MQ=255)
                                                                                                                                                                                                            ggCCCGAACCAGACGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGTATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGGAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCa    <  1:116524/216‑1 (MQ=255)
                                                                                                                                                                                                                        |                                                                                                                                                                                                             
TCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCC‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATA  >  NC_000913/2734368‑2734788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑