Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,156,084	G→A	V244I (GTC→ATC)	yqhD →	NADPH‑dependent aldehyde reductase YqhD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,156,084	0	G	A	100.0%	15.0 / NA	8	V244I (GTC→ATC)	yqhD	NADPH‑dependent aldehyde reductase YqhD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

GCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTAATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGCGGCGACTCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCTAAGCTGCTGCAATATGCTG  >  NC_000913/3155919‑3156279                                                                                                                                                                      |                                                                                                                                                                                                    gCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTAATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACATCATGTGGGCGGCGACTCAGGCGCTGAACGGTTTGATTGGCGCTGTCTTACCGCAGGACTggg                                                                                                                                      <  1:194118/229‑1 (MQ=255)                                                              aTGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTCCTGGCGCTAATCGACGATGCTCCGACTACCCTGGAAGAGCCAGAAAACTACGAAGTGAGCGCCAACATCATGTGGTCGGCGAATCAGGCGATGAACGGCTTGATTGGCGCTGGCGTACCGCAGTACTgg                                                                                                                                       >  1:244687/1‑167 (MQ=255)                                                                                gNTTCGCAGAAGGCATTTTGCTGACGCTAATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACATCATGTGGGCGGCGACTCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAt                                 >  1:198970/1‑251 (MQ=255)                                                                                                                gAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGCTGTGCACGACAACATCATGTGGGCGGAGACTCCGGCGCTGCACGGTTTGCTTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGCACTGCCTGCGATGCACGGTCTGGCTCACGCGCAACCACTGGCTATCGTCCTGCCTGCACTGCGGCATGAAAAACACGATACCAAGCGCGCTACGCTGCTGCAATATGCta  >  1:193311/1‑249 (MQ=255)                                                                                                                gAAGANGGTCCNANNGCCCTGAAAGTGCCAGAAANCTACGATGTGCGCGCNAACANCATGTNggng                                                                                                                                                                                          >  2:110497/1‑66 (MQ=255)                                                                                                                                cccTGAAAGAGCCAGAAAACTACGAGGTGCGCGCCAACATCATGTGGGCTGCGACACAGGCGCTGAACGGTGTTATTGGCGCTGGCGTACCGCAGGACTGGGCaa                                                                                                                                   <  1:103503/105‑1 (MQ=255)                                                                                                                                          gCCAGAAAACTACGATGTGCGCGCCAACATCATGTGGGCGGCGACTCAGGCGCTGAACGGTTTGATTGGCGCTGGCg                                                                                                                                                     >  1:8406/1‑77 (MQ=255)                                                                                                                                          gCCAGAAAACTACGATGTGCGCGCCAACATCATGTGGGCGGCGACTCAGGCGCTGAACGGTTTGATCGGCGCTGGCg                                                                                                                                                     <  2:8406/77‑1 (MQ=255)                                                                                                                                                                      |                                                                                                                                                                                                    GCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTAATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGCGGCGACTCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCTAAGCTGCTGCAATATGCTG  >  NC_000913/3155919‑3156279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑