Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,441,396	G→A	L94L (CTG→TTG)	rpsD ←	30S ribosomal subunit protein S4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,441,396	0	G	A	100.0%	14.1 / NA	7	L94L (CTG→TTG)	rpsD	30S ribosomal subunit protein S4
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/4);  total (3/4)

CGAAGCCCATACGGTATACAACGTTGTCCAGACGACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGAC  >  NC_000913/3441354‑3441638                                           |                                                                                                                                                                                                                                                   cGAAGCCCATACGGTATACAACGTTGTCCAGACGACCTTCCAACAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACGTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCATGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACg                                    <  1:168488/251‑1 (MQ=255)      cccctACGGTATACAACGTTGTCCAGACGACCTTCCAACAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGcc                                  >  1:91328/5‑248 (MQ=255)             ggTATACAACGTTGTCCAGACGACCTTCCAACAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACACTCCGGCACACTATAGATACTGCGAACGTTTTGCTTTTCACGCAGCGGCACACCAGAGTCAGACAGACGCGGTTCACGCGCACCCTCGTGGCCAGTGGCGTGTTCAATTTGACACTTGGTATCGATCGCGCGAACGCCAGACTTAAgg                        <  1:243946/251‑1 (MQ=255)                            ccAGACGACCTTCCAACAGAGCCAACAGGTTTTCACCGGTGCTGCCTTTCAGACGTGCTGCATCTTTGTAGTAGTTACGAAACTGCCGCTCCAGCACCCCATAGATCCGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGCCAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGccc         >  1:186543/1‑251 (MQ=255)                             cAGACNCCCTTNCNNCAGCGCCAACTGGTTTTCANCGGTGTTGCCTTTCANACGTNCTGctnccntn                                                                                                                                                                                                >  2:96938/1‑63 (MQ=255)                             cAGACGACCTTCCAACAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATcgcgc                                         <  1:96938/218‑1 (MQ=255)                                   acCTTCCAACAGAGCAAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTTCTTCTTTGTAGTCGTTACGGCACTCACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGTAACTGCACACCATCGTCAGACAGATGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTGGTTCACTTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTAacgac  >  1:242569/1‑251 (MQ=255)                                    ccTTCCAACAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGcttctt                                                                                                                                                                                                  <  1:127220/58‑1 (MQ=255)                                           |                                                                                                                                                                                                                                                   CGAAGCCCATACGGTATACAACGTTGTCCAGACGACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGAC  >  NC_000913/3441354‑3441638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑