Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	723,973	C→T	E148K (GAA→AAA)	kdpD ←	sensor histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	723,973	0	C	T	100.0%	40.2 / NA	16	E148K (GAA→AAA)	kdpD	sensor histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/9);  total (7/9)

TGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCGGGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTCCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATG  >  NC_000913/723749‑724150                                                                                                                                                                                                                                 |                                                                                                                                                                                  tGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAACATGTTCCATGGCGCGCTCCGCCTGCCAGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCGGGGGCAAGTCCAACAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGAGACGCCGCTGACCAAATCATTCAGACTTTTAAGATGCTGAACGTTGACGGTAGTg                                                                                                                                                          >  1:241372/1‑250 (MQ=255)                                   cGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCGGGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAAGTCCGGTGACGCCGCTGACCACATCATTCAGACTTTTCAGATGCTGAACGTTGAc                                                                                                                                                                 <  1:58624/209‑1 (MQ=255)                                    ggAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCAGGGGGCATGTCCACCAGCACCACGTCGTCGGCGGCATCGAACAAAGGATCGGGCACGCTTTACCGTACCAGAATTCCGGCGACGCCGCTGACCACATCATTCAGACTTTTCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGat                                                                                                                       >  1:142708/1‑250 (MQ=255)                                     gAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCACTATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCGGGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTTCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCcagcag                                                                                                                                >  1:127508/1‑240 (MQ=255)                                      aaaaaaTGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACGTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCGGGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTTCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATc                                                                                                                    <  1:108203/251‑1 (MQ=255)                                                                ntGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCGGGGGCAAGTCCACCCGCACCACGTCGTCGGCGGCATCGAACCACGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCCGCCTTTTCAGATGCTGAACGTTGCCGGTCGTGAAAACATCACTGCCATCTTCCAGCAGTTCTTCGATATCCTGCCCGCGTTTGGGATGCCGGGAAc                                                                                          >  1:33463/2‑251 (MQ=255)                                                                                                            gATCGTCCGGGGGCAAGGCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTTCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATg                                                                                                                                              <  1:85721/155‑1 (MQ=255)                                                                                                                    nggggCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTTCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGgcggcg                                      >  1:203987/2‑251 (MQ=255)                                                                                                                                             cgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTTCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTTGGATGACTGGATCCGGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAAc             <  1:36175/251‑1 (MQ=255)                                                                                                                                                         cGAAAAAAGGATAGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTTCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTGTGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATg  <  1:40414/250‑1 (MQ=255)                                                                                                                                                          gAAAAAAGNANNNGNNACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCNTTCAGACTTTTCAGANg                                                                                                                                                                             >  2:173111/1‑78 (MQ=255)                                                                                                                                                          gAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTTCAGATg                                                                                                                                                                             <  1:173111/78‑1 (MQ=255)                                                                                                                                                                                           aaTTCCGGTGACGCCGCTGACCACATCATTCAGACTTTTCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTggn                                                                                     <  1:153424/133‑2 (MQ=255)                                                                                                                                                                                           aaTTCCGGTGACGCCGCTGACCACATCATTCAGACTTTTCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGgc                                                                                     >  2:153424/1‑133 (MQ=255)                                                                                                                                                                                                 ggTGACGCCGCTGACCACATCATTCAGACTTTTCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGc                                                                                                                <  1:123766/100‑1 (MQ=255)                                                                                                                                                                                                                     cntNCATACTTTTCAGATGCTGAACGTTGACGGTAGTGAAAANATCANTNNCANCNTCnagcag                                                                                                                                <  2:127508/64‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                  TGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCGGGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCACATCATTCAGACTTTCCAGATGCTGAACGTTGACGGTAGTGAAAACATCAATGCCAGCTTCCAGCAGTTCTTCGATATCCTGCCAGCGTTTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATG  >  NC_000913/723749‑724150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑