Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,705,723	G→A	L468L (CTG→TTG)	lepA ←	30S ribosomal subunit biogenesis factor LepA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,705,723	0	G	A	100.0%	19.9 / NA	8	L468L (CTG→TTG)	lepA	30S ribosomal subunit biogenesis factor LepA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/6);  total (2/6)

ACGCAGCTGTTTCACGGTGGATCGCGCAATGATGTGCGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTC  >  NC_000913/2705487‑2705912                                                                                                                                                                                                                                             |                                                                                                                                                                                              aCGCAGCTGTTTCACGGTGGATCGCGCAATGATGTGCGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGGAATCCAAAGACGCATAACCAc                                                                                                                                                                                 <  1:214989/251‑1 (MQ=255)                                                                                                       caccaACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAAAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTAcn                                                                                                                  <  1:77017/212‑2 (MQ=255)                                                                                                                         gTTTTGCGAATTATCACGGCGGGTGATACGCGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCCAAGCCGCATAACCACGCGAGGCAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGCTCTCGTACGTCAGCGCCACCTGATTACCGTGGTACACCATATTGGTCTGCACGCCGCGTTTTTCt                                                          >  1:80272/1‑250 (MQ=255)                                                                                                                                    tATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAAAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGt                                               <  1:147691/250‑1 (MQ=255)                                                                                                                                                                  cAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGCAGTTGTAATCCAAAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCCCGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgcagc                >  1:183829/1‑251 (MQ=255)                                                                                                                                                                                 tGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCTCTTGAAGTTGTAATCCAAAGACGCATAACCACGCGAGGTAGATTTCTGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTGTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACActc  <  1:221225/250‑1 (MQ=255)                                                                                                                                                                                                                        aGCGCTTGAAGTTGTAATCCAAAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTgn                                                                              <  1:76985/135‑2 (MQ=255)                                                                                                                                                                                                                                      tAATCCAAAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTg                                                                               <  1:139330/120‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                              ACGCAGCTGTTTCACGGTGGATCGCGCAATGATGTGCGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTC  >  NC_000913/2705487‑2705912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑