Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,353,758	T→A	D254V (GAT→GTT)	sapC ←	putrescine ABC exporter membrane protein SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,353,758	0	T	A	100.0%	19.0 / NA	9	D254V (GAT→GTT)	sapC	putrescine ABC exporter membrane protein SapC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (4/5);  total (4/5)

CCGCGGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCC  >  NC_000913/1353519‑1353964                                                                                                                                                                                                                                                |                                                                                                                                                                                                               ccGCTGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGTCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCAtn                                                                                                                                                                                                     <  1:31025/251‑2 (MQ=255)                         gTCATACTTACCCGGTCGACGGCTTTAACCCACTCATAACCGGTTTTAAATTCAATGGTAAGGTTACGAATATCGAGTAATGGCATTATTCAACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCAAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAAAACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGt                                                                                                                                                                             >  1:96606/1‑251 (MQ=255)                                                                                  gTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAAACGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCGCCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGc                                                                                                                                                       <  1:179498/216‑1 (MQ=255)                                                                                                        aTGGCATTNTNNNANNGCCGCAATAATCGCACGACGAAAGCCATCGCCAAGGAGGTTAACNAACAACACGATAATCNTANTTGCNGCANCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCCTTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGACAGCNAAgng                                                                                                                                                 >  2:181787/1‑200 (MQ=255)                                                                                                        aTGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTTGCAGCATGACAGTCCACGGGGCGACATAAATCATTTCCATCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAgcg                                                                                                                                                 <  1:181787/200‑1 (MQ=255)                                                                                                                                                      ccAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACTGTGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGcc                                                                                                                                                                     <  1:120821/134‑1 (MQ=255)                                                                                                                                                       cAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGAATGACAGTCAACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATc                                                                                                                                                               >  1:227704/1‑139 (MQ=255)                                                                                                                                                                                                     nagcaTGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAAACAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTcc  >  1:63730/2‑250 (MQ=255)                                                                                                                                                                                                          tGACAGGCCACGGGGCGACATAAAGCAGGTCCAGCGCAACACCGAGCAGCCCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCga                                                                                                                                                             <  1:134864/90‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                               CCGCGGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCC  >  NC_000913/1353519‑1353964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑