Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	676,374	C→T	E58E (GAG→GAA)	ybeQ ←	Sel1 repeat‑containing protein YbeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	676,374	0	C	T	100.0%	30.3 / NA	12	E58E (GAG→GAA)	ybeQ	Sel1 repeat‑containing protein YbeQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/5);  total (7/5)

TCTGATACCAGACAACCGCTTCTTTATAATTGGGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGATAATCTCATCTATTGATAAATTATCGCAGCAACTTGACGTGAAAATCATTATCATTTCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTTGCGTAAGCCTTATTT  >  NC_000913/676175‑676619                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      tCTGATACCAGACAACCGCTTCTTTATAATTGGGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTGTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGTTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTtcg                                                                                                                                                                                                                   <  1:235059/236‑1 (MQ=255)                                                             cAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGTTCAGCGGCCAgn                                                                                                                                                                                                                                           <  1:156389/152‑2 (MQ=255)                                                                   cGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGTCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGTTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTTCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGc                                                                                                                                   <  1:188247/250‑1 (MQ=255)                                                                                                                 gaagaTGGCTTGCTCATTATCTTTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTACGGAATACTGTGCTTCACAATGACCTTGTTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTCTCGCGATTATAATAAAAc                                                                                                                                                                         >  1:135906/1‑166 (MQ=255)                                                                                                                  aagaTGGCTTGCTCATTATCGTTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGTTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCGATCGCGATTATAATAAAACCCAACAATATAGGGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGATAATCTCATCTATTGATAAATTATCGCAGCAACTTGACgn                                                                                   <  1:54077/251‑2 (MQ=255)                                                                                                                             cTCATTATCTTTATGACGGCTTTTCTCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGTTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGATAATCTCATCTATTGATAAATTATCGCAGCAACTTGACGTGaaa                                                                               >  1:79478/1‑244 (MQ=255)                                                                                                                                               gCTTTTATCCACGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGTTCAGCGGCCAGCTGCAGCCAGTAAAAGGCCTTTTCGTCGTCCGGAGAAACAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGATAATCTCATCTATTGATAAATTATCGCAGCAACTTGACGTGAAAATCATTATCATTTCCTTATGGTTGc                                                      <  1:165999/251‑1 (MQ=255)                                                                                                                                                                       cccTAAGGAATACTGTGCTTCACAATGACCTTGTTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGATAATCTCATCTATTGATAAATTATCGCAGCAACTTGACGTGAAAATCATTATCATTTCCTTATGGTTGCAGATAAAACATATTATATTTTTAt                              >  1:96271/1‑251 (MQ=255)                                                                                                                                                                       cccTAAGGAATACTGTGCTTCACAATGACCTTGTTCAGCGGACAGCTTCCGCCAGTAAAAGGCTTTTTCGTAGTCCGGAGAATAACTTGCGCTATCGCGATTATACTAAAACCCAACAATATATTGAGCCTCGCAATCGACTTTTTCAGCACGTTCGATAATCTCATCTATTGATAAATTATCGCAGCCACTTGACGTGAAAATCATTATCATTTc                                                                 >  1:171837/1‑216 (MQ=255)                                                                                                                                                                                    tgtgCNTAAGANTNNCCTTCTTCAGTGGCCAGCTNCAGCCAGTAAAAGGCNTTTTNGTCGTNCGna                                                                                                                                                                                                          >  2:104112/1‑66 (MQ=255)                                                                                                                                                                                       gCTTCACAATGACCTTGTTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGATAATCTCATCTATTGATAAAt                                                                                                      >  1:105347/1‑163 (MQ=255)                                                                                                                                                                                                   ccTTGTTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGAGATTATAATAAAACCCAACAATATATTGAGCCTCGAAATCGCCTTTTTCAGCACGTTCGATAATCTCATCTATTGATAAATTATCGCAGCAACTTGACGTGAAAATAATTATCATTTCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTTGCGTAAGCCTTAttt  >  1:117028/1‑251 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TCTGATACCAGACAACCGCTTCTTTATAATTGGGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGATAATCTCATCTATTGATAAATTATCGCAGCAACTTGACGTGAAAATCATTATCATTTCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTTGCGTAAGCCTTATTT  >  NC_000913/676175‑676619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑