Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,218,459	C→T	intergenic (+46/‑137)	aceA → / → aceK	isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,218,459	0	C	T	100.0%	21.4 / NA	10	intergenic (+46/‑137)	aceA/aceK	isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/5);  total (5/5)

GCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGATGAGGGCGCTAAATGCCGCGTGGCCTGGAATTATTGATTGCTCAAACCATTTTGCAAGGCTTCGATGCTCAGTATGGTCGATTCCTCGAAGTGACCTCCGGTG  >  NC_000913/4218216‑4218686                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    gCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTAGTCAGGGCGGCACGTCTTCAGTCACCGCTCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGTCGCATc                                                                                                                                                                                                                               <  1:184976/250‑1 (MQ=255)                                          gcagcCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGTCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCt                                                                                                                                                                                     >  1:88079/1‑251 (MQ=255)                                                        ccgccgCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAATTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCATTGCTGACTGTAGGCCGGATAAGGCGTTCACGTCGCATCCGGCAATCGGTGCACGATTCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAg                                                                                                                                                                       <  1:216146/251‑1 (MQ=255)                                                                       gCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGTCGAATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGt                                                                                                                                                         >  1:168880/1‑250 (MQ=255)                                                                                          ccAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGGTGCTGACTGTAGGCCGGATAAGGCGTTCACGTCGCATCCGGCAATCGGTGCGCGAGGCCTGATGGGACGCTGTCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGc                                                                                                                                           <  1:116063/245‑1 (MQ=255)                                                                                                             ggTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGGAGGCCGGATAAGGCGTTCACGTCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGc                                                                                                                                                                             <  1:87680/192‑1 (MQ=255)                                                                                                                                                 ggcACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGTCGCATCCGGCAATCGGTGCACGATGCCTGATGCNACGNNNnngcnnnnnn                                                                                                                                                                                   >  2:157998/1‑144 (MQ=38)                                                                                                                                                           cAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGTCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGATGAGGGCGCTAAATGCCGCGTGGCCTGGAattattn                                                                     <  1:157998/250‑2 (MQ=255)                                                                                                                                                                                                        caacaacaaCCGTTGCTGACTGTAGGCAGGATAAGGCGTTCACGTCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGATGAGGGCGCTAAATGCCGCGTGGCCTGGAATTATTGATTGCTCAAACCATTTTGCAAGGCTTCGATGCTCAGTATGGTCGAt                       >  1:200652/1‑251 (MQ=255)                                                                                                                                                                                                                             ntAGGCCGGATAAGGCGTTCACGTCGCATCCGGCAATAGGTGAACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTCCAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCACTTCTCTGCTCCTGATGAGGGCGCTAAATGCCGCGTGGCCTGGACTTATTGATTGCTCAAACCATTTTGCAAGGCTTCGATGCTCAGTATGGTCGATTCCTCGATGTGACCTCCGGTg  >  1:47379/2‑251 (MQ=39)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    GCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGATGAGGGCGCTAAATGCCGCGTGGCCTGGAATTATTGATTGCTCAAACCATTTTGCAAGGCTTCGATGCTCAGTATGGTCGATTCCTCGAAGTGACCTCCGGTG  >  NC_000913/4218216‑4218686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑