Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,838,600 C→T W222* (TGG→TAG)  ascG ← DNA‑binding transcriptional repressor AscG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,838,6000CT100.0% 33.9 / NA 14W222* (TGG→TAG) ascGDNA‑binding transcriptional repressor AscG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/8);  total (6/8)

GAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGCTATGGCTGCTGTTTTTGCGC  >  NC_000913/2838367‑2838812
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                    
gAATCAGCTCAGTCACCGGAATTTTTACGCTGTAGAGCTCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGTCACCGCTACGCCGCGCTCGAGTAATGCGTTCATCGCTCCGTTCGCCGTGTCGTCGTTACTGGGATCCAACGCGCGAAATTGAGGCCCGCGTTCGGGTAACTTTTCTACCCCTGCGGCCCCGCTCGCAGGCGTCTATTTACCGTTAGCGa                                                                                                                                                                                                       <  1:130373/249‑1 (MQ=255)
                               ggAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCTCGTTCGAGCAACATTTCTTCCCCTGCGGCCCCGCTGGCAGGCGTCTATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTgng                                                                                                                                                                      <  1:199333/251‑1 (MQ=255)
                                    tcgcCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCATCTATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAgc                                                                                                                                                                 >  1:56770/2‑251 (MQ=255)
                                                                                                  ggCNCNNCNNCGCNGCGCTCGTGTAACGCTTTCATNNCACCTATCGNCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCANCATTTCTACNNCTNCGGCCNCNNNGNNNGGNATCTATTGACCGTTAGCGANAANNTTTTCANTGAGCGCAATACCATGCTGCGCCAgc                                                                                                                                                                 <  2:56770/189‑1 (MQ=255)
                                                                                                   gCACCGCTACGCCGCGCTCGTTTAACGCTTTTATTGCACCTATCGCCATAACGTCGTTACGGGCAACGAACGCGCAAAATTTAGCCCCACGTTCGAGCAACATTTCTCCCCCCTCGGCCCCGCTGGCAGGCGTCTATTTACCGTTAGCGAGAAGTTTGTCATTGAGCGCAATACCATGCTGCGCCAGCGTCTCTTTATACCCGTCAAGACTTTCAATGCTGGTggg                                                                                                                           <  1:90938/226‑1 (MQ=255)
                                                                                                                      gtgtAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCTCGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCTATTTGCCGTTAGCGATAAGTTTTTCATTGAGCGCAAGACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTGCAATGCtggtg                                                                                                                             <  1:165205/205‑1 (MQ=255)
                                                                                                                                                                ngCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCACCATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCTATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGACCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTAt                                     >  1:53822/2‑251 (MQ=255)
                                                                                                                                                                     cTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGAAGGCGTCTATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCACTACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCACTCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGt                                         >  1:126833/1‑242 (MQ=255)
                                                                                                                                                                       aaCGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCGACCCCTTCGGCCCCGCTGGCAGGCGTCTATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTgcgn                                                                                                                                                                     <  1:47049/116‑2 (MQ=255)
                                                                                                                                                                             ntAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCTATTTACCGTTAGCGACAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGcc                                                              >  1:149940/2‑213 (MQ=255)
                                                                                                                                                                                         ccACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCTATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCg                                                                                >  1:19629/1‑183 (MQ=255)
                                                                                                                                                                                                   gCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCTATTTACCGTTAGAGATAAGTTTTTCATTAAGCGCACTACCATGCTGCGCAAGCCCGTCTTTATACCCGGCAAGACATTCCATGCTGGTGGGGGAATCCATCGAGCAGGTAAGGAAAGCACTATCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAAATGGTCTGTTTCTGATCGCACCAGACGCTATGGCTGCTGTTTTTgcgc  >  1:179738/1‑251 (MQ=255)
                                                                                                                                                                                                     aaCATTTCTACCCCATCGGCCCCACTCTCAGGCGTCTAGTGACCGTTAGCTATAAGTTTTCCATTGAGCGCAATACCACGCTGCGCCTGCGCGTCTGTATACCCTGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCgg                                                                               <  1:252063/172‑1 (MQ=255)
                                                                                                                                                                                                               ccccTTCGGCCCCGCTGGCAGGCGTCTTTTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATCCCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCa                                                                                                                   <  1:121533/126‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                    
GAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGCTATGGCTGCTGTTTTTGCGC  >  NC_000913/2838367‑2838812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑