Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,366,530	G→A	R82C (CGT→TGT)	dcuA ←	C4‑dicarboxylate transporter DcuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,366,530	0	G	A	100.0%	31.9 / NA	12	R82C (CGT→TGT)	dcuA	C4‑dicarboxylate transporter DcuA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/3);  total (9/3)

ACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATG  >  NC_000913/4366322‑4366642                                                                                                                                                                                                                 |                                                                                                                 aacACTGCCGCTGAGATTGGCGATGCGGTGAGCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTGCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGTTAGTCAGGAAATAGGTCACGATCGGGGCGAGGATCTGGATGTATTTCGGGTTACAGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGAcc                                                                        <  1:172176/249‑1 (MQ=255)       gCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACAGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGc                                                                  >  1:117931/1‑251 (MQ=255)          gCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGAGCGAGGATCGTGATGTATTTCGGGTTACAGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAAc                                                               >  1:14766/1‑251 (MQ=255)                    gagATGCGGTGATCGCAATCTGCGCGGATACGCCTGCAGTCGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCACATTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACAGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGc                                                      >  1:205153/3‑250 (MQ=255)                                           ncgGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACAGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGt                                                                               >  1:155113/2‑202 (MQ=255)                                                                                  aCGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAACTAGGTCAAGATCGGAGCGAGGATCGTGATGTATTTCGGGTTACAGCGAAGAAGCTTTTATGTCTGATGAACCCGATAGTCCAGACCGCCAGCAAc                                                               >  1:183294/1‑179 (MQ=255)                                                                                     ccTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACAGCGCAACANCttnn                                                                                                    >  2:155443/1‑137 (MQ=255)                                                                                     ccTTGTTCCTGCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACAGCGCAACAGCTTTTCTGTCTGATGAACCAGATAGTCCAGan                                                                         <  1:155443/166‑2 (MQ=255)                                                                                      cTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTAACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACAGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATg                             >  1:71886/1‑209 (MQ=255)                                                                                               nnnnnncTNNNNNGATNACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACAGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGt                                                                               <  2:155113/144‑1 (MQ=255)                                                                                                                                                                                         ggATCGTGATGTATTTCGGGTTACAGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGNATg  >  2:158041/1‑137 (MQ=255)                                                                                                                                                                                                          gggTTACAGCGAAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGAc               >  1:211470/1‑107 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                 ACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATG  >  NC_000913/4366322‑4366642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑