Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	541,644	C→T	L360L (CTC→CTT)	ybbY →	putative purine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	541,644	0	C	T	93.3%	33.5 / ‑3.5	15	L360L (CTC→CTT)	ybbY	putative purine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (5/9);  total (5/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

GCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTACCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAATGGCATTACCGCCTGTGTATCTGCAAGACCTTCCATTAACGCTTCGTCCTCTGCTCAGTAACGGCTTATTGGTCGGGATTTTACTGGCTGTTCTTATGGATAACCTTATTCCGTGGGAACGCATCGAATAATTTGTTG  >  NC_000913/541502‑541873                                                                                                                                               |                                                                                                                                                                                                                                      gCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTACCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTTTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCgg                                                                                                                                                       <  1:10623/223‑1 (MQ=255)        cacGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTACCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTTTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAATgg                                                                                                                                         <  1:92427/230‑1 (MQ=255)                        atttatGGCAGCGTTATTTGCCTGCTGGTTGCGCTGGTTCCTGCACTCACGCTACTGTTTTGCAGTATCCCTTTACCCGTGAGTATTGCTGTCATGCTGGTTTCTTATCTGCCTTTGCTTTTTTCCGCGCTGGTGGTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTAt                                                                                                                                                                                 <  1:228886/174‑1 (MQ=255)                                     ttATTTGCCTGCTGGTGGCGCTGGTTCCTGCGCTCTCGCGACTGTTTTTCATGATCCCTTTACCCGTGGGTAGGGCGGTCATGCTGGTTGCGTATCTGCCTTTACTTTTTTCCGCGCTGGTGTTTAGcaa                                                                                                                                                                                                                <  1:235113/130‑3 (MQ=255)                                                             ttCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTACCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTTTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAATGGCATTACCGCCTGTGTATCTGCAAGACCTTCCATTAACGCTTCGTCCTCTGCTCAGTAACGGCTTATTGGTCgg                                                                <  1:59932/250‑1 (MQ=255)                                                                  gCACTCACGCGCCTGTTTTGCAGTATCCCTTTACCCGTGCGTAGTGCGGTCCTGCTGGTTTATTCTCTGCCTTTACTTTTTTCCGCGCTGGTGTTTAGCAAGCAAATAAAGTTTACCGCTAGCCATATTTATCGACTCGCATTGACGTTATTTGTCGGCATATTTTTAATGGCATTACCGCCTGTGTATCTGCAAGACCTTCCATTACCGCTTCGTCCTCTGCTCAGTAACGGCTTATTGGTCGGGCtttt                                                          >  1:170099/1‑251 (MQ=255)                                                                        aCGCGACTGTTTTGCAGTATCCCGTTACCCGTGATTAGTGCGGTCAGGCTGGTTTCTTAGCGGCATGTAGGCTTTTCCGCGGTGGTGTTGAGCCAGCAAATAACGTGTACCGCGCGCAATATTAAACGACTCGCATTGCGGTTATTTGTCGTCATATGTATAAAGGGATGGCCGCCTGTGTATCTGCAAGACCTTCCATTAACGCTTCGTCCTCTGCTCAGTAACgg                                                                            <  1:165509/227‑1 (MQ=255)                                                                           cgACTGTTTTGCAGTATCCCTTTACCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTTTTTTCCGCGCTgn                                                                                                                                                                                                                          <  1:149406/82‑2 (MQ=255)                                                                           cgACTGTTTTGCAGTATCCCTTTACCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTTTTTTCCGCGCTgg                                                                                                                                                                                                                          >  2:149406/1‑82 (MQ=255)                                                                                          aTCCCTTTACCCGTGATTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTTCTTTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAATGGCATTACCGCCTGTGTATCTGCAAGACCTTCCATTAACGCTTCGTCCTCTGCTCAGTAACGGCTTATTGGTCGGGATTTTACTGGCTGTTCTTATGGATAAcn                                   <  1:52445/250‑2 (MQ=255)                                                                                                 tACCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTTTTTTCAGCGCTGGTGTTTAGCCAGCAAATAACGTTAACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAATGGCATTACCGCCTGTGTATCTGCAAGACCTTCCATTAACGCTTCGTCCTCTGCTCAGTAACGGCTTATTGGTCGGGATTTTACTGGCTGTTCTTATGGATAACCTTATTCCg                           >  1:130971/1‑251 (MQ=255)                                                                                                     cGTGAGTAGTGCGGTCATGCTGTTTTCTTATCTGCCTTTACTTTTTTCCGCGCTGGTGTTTAGCCAGCAAATATGGTTTCCCGCTCTCAATATTTATCGACACGCATTGCCGTAATTTGTCGGCAAATTTTTAATGGCATTGCCGCCTGTGTATCTGCAAGACCTTCCATTAACGCTTCGTCCTCTGCTCAGTAACGGCTGATTGGTCGGGATTTTACTGGCTGTTCTTATGGATAACCTTATTCCGTg                         <  1:9374/249‑1 (MQ=255)                                                                                                                          ggTTTCTTATCTGCCTTTACTTTTTTCCGCGCTGGTGTTGAGCCAGCAAATAACGGTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTAGTTGTCGGCATATTTTAAAGGGCATGACCGCCTGTGTATCGGCAATACCTTCCATTAACGCTTCGTCCTCTGCTCAGTAACGGCTTATTGGTCGGGATTTTACTGGCGGTTCTTATGGATAACCGTATTCCGTGGGAACGCATCGAATAATttgttn  <  1:25426/251‑2 (MQ=255)                                                                                                                              tCTTATCTGCCTTTACTTTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTNNTTTGTCNNc                                                                                                                                                      >  2:15777/1‑99 (MQ=255)                                                                                                                                       ccTTTACTTTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTAGAAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAATGGCATTACCGCCTGTGTATCTGCAAGACCTTCCATTAACGCTTCGTCTTCTGCTCAGTAACGGCTTATTGGTCGGGATTTTACTGGCTGTTCTTATGGATAACCTTATTCCGTGGGAACGCATc               >  1:249970/1‑225 (MQ=255)                                                                                                                                               |                                                                                                                                                                                                                                      GCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTACCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAATGGCATTACCGCCTGTGTATCTGCAAGACCTTCCATTAACGCTTCGTCCTCTGCTCAGTAACGGCTTATTGGTCGGGATTTTACTGGCTGTTCTTATGGATAACCTTATTCCGTGGGAACGCATCGAATAATTTGTTG  >  NC_000913/541502‑541873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑