Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,616,014	G→A	K84K (AAG→AAA)	yneK →	protein YneK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,616,014	0	G	A	100.0%	17.7 / NA	8	K84K (AAG→AAA)	yneK	protein YneK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

GAGTGGCGCACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTC  >  NC_000913/1615895‑1616197                                                                                                                        |                                                                                                                                                                                        gagTGGCGCACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAAGAACCTTGACAACTTATCATATATTCCGAGGACTTCTTTTCGTGGTAACGAGATCCTATGCAATGAAAAAGTTTAGTTGAAACTCACTTATCCCGGCAGGAGTGGAAGACACAAAGAAAATTTTGAAGTTCAGGTAGTAATAAATTTAAGTTCTATGTGTTGAGATAATGTTCGCCATGATGGAGAAATTAATAttttttn                                                      <  1:47624/251‑2 (MQ=255)                               nccACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAAGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATggg                        >  1:151325/2‑251 (MQ=255)                                 nacaAAAACGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAAGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTc                                                                                  >  1:68929/2‑191 (MQ=255)                                                     aCCTTGACAGCTTATCATATATTCAGAGGAATTCTTTTCGGTGTAACGACACGCTATACAATGAAAAAGTTCAGTTGAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATAGTGAATTTCAGGTAGTAATAAATTTAATCCCGATTTATTTAGATAATTTTCTCCATGATGGAGAAATGAATATTTTTTGCTGTCCCAACCCAGTGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTc  <  1:190663/251‑1 (MQ=255)                                                                                   cttcttTTCGGGGTAACGACACGCTATACAATGAAAAAGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGACGACCCAAAGAAAATATTGCATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATAtttttt                                                       >  1:106331/1‑168 (MQ=255)                                                                                                           tataCAAGGAAAAAGTTCAGTTTAAACTCAGTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTAGTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGgcg                     <  1:16443/178‑1 (MQ=255)                                                                                                                 aTGAAAAAGTTCAGTTTAAACTCACTTAGCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGgcgc                    <  1:228383/173‑1 (MQ=255)                                                                                                                   gAAAAAGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTACTATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATgggg                       >  1:84360/1‑168 (MQ=255)                                                                                                                        |                                                                                                                                                                                        GAGTGGCGCACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTC  >  NC_000913/1615895‑1616197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑