Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,598,800	G→A	G214G (GGC→GGT)	opgB ←	phosphoglycerol transferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,598,800	0	G	A	100.0%	56.4 / NA	20	G214G (GGC→GGT)	opgB	phosphoglycerol transferase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/8);  total (12/8)

CGCTTTTCAGCTCTTCTGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCAACGCGCCCAGTTCAGGCGTGAGATCCGGGAAAGCCTCGTTATCAAAATAGGTCCGCTCGAGACTTTCGCCGTAGATATAAACCAGGTTGAGTTTCGGGTCAGGGATAGTTTTCGACGGCTCTTTATAATAAGCCGCAAAGTCCGGGTCGCCGTCGCGTGACTG  >  NC_000913/4598571‑4599030                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       cGCTTTTCAGCTCTTCTGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGACCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCGGGAAGAAGCTGGAGAAAGAGGCGGAGGAGTTGCCTTCAAAGGGGGCAAACAGCGGGATGCCGCACTGAGAAGCCACCAAAAACGCAATCGTGTAATCTGtt                                                                                                                                                                                                                   >  1:2152/1‑251 (MQ=255)                                            ngACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTAGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCGGGAAGAAGCTGGAGCCAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCCGTTCCCGGCAGCTGATGCGTGTGGc                                                                                                                                                                                             >  1:28115/2‑230 (MQ=255)                                                    ngAACCCATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCAAcgcg                                                                                                                                                                >  1:77616/2‑251 (MQ=255)                                                    ngAACACATCTTTACCGGCAAAACGCGGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCAAcgcg                                                                                                                                                                >  1:153656/2‑251 (MQ=255)                                                                                        ccTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCTGGAAGAAGCTGGAGACAGAGGCGTAGGCGTTGCCTTCAAAGTGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTTTGGCTGAAATCCAGGCCGTCATTTTTCAAc                                                                                                                                                                   <  1:197431/212‑1 (MQ=255)                                                                                           gCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAgg                                                                                                                                                                                  <  1:224540/194‑1 (MQ=255)                                                                                              cGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCAACGCGCCCAGTTCAGGCGTGAGATCCGGGAAAGCCTCGTTATCaaa                                                                                                                       >  1:140481/1‑250 (MQ=255)                                                                                                                 ctaGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCGGGGAGAAGCTGGAGACAGAGGCGGCGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCAACGCGCCCAGTTCAGGCGTGAGATCAGGGAAAGCCTCGTTATCAAAATAGGTCCGCTCGAGACttt                                                                                                   >  1:229468/3‑251 (MQ=255)                                                                                                                                 tctcCCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCAACGCGCCCAGTTCAGGCGTGAGATCCggg                                                                                                                                        <  1:2902/198‑1 (MQ=255)                                                                                                                                                    gCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCATAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCTTTCATTTTTCAACGCGCCCAGTTCAGGCGTGAGATCCGGGAAAGCCTCGTTATCAAAATAGGGCCGCTCGAGACTTTCGCCGTAGATATAAACCAGGTTGAGTTTCGGGTc                                                                 <  1:218946/250‑1 (MQ=255)                                                                                                                                                              nntGNNNNNAGANGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCAACgcgc                                                                                                                                                               <  2:153656/144‑1 (MQ=255)                                                                                                                                                                                      gCCTTCAAAGGGGGAAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGc                                                                                                                                                                                             >  1:72612/1‑92 (MQ=255)                                                                                                                                                                                       ncTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCAACGCGCCCAGTTCAGGCGTGAGATCCGGGAAAGCCTCGTTATCAAAATAGGTCCGCTCg                                                                                                          >  1:49894/2‑174 (MQ=255)                                                                                                                                                                                       ccgtCAAAGGGGGCAAACAGCTGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTc                                                                                                                                                                      <  1:163580/111‑1 (MQ=255)                                                                                                                                                                                        cTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCAACCATATCCGCAATCGTGGAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCACCGCGCCCAGTTCAGGCGTGAGATCCGGGAAAGCCTCGTTATCACAATAGGTCCGCTCGCGACTTACGCCGTAGATATAAACCAGAGTGAGTTTCGGGGCAGGGATAGTTTTCGACGGCTCTTTATAATAAGCCGc                             >  1:530/1‑250 (MQ=255)                                                                                                                                                                                        cTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCAACCATACCCGCAATCGTGTAATCTGTTACGGACAGCTGCTGCGTGAGGCTGAAATCCAGGCATTCATTTTTCAACGCGCCCAGTTCAGGAGTGAGATCCGGGAAAGCATCGTTATCAAAATAGGTc                                                                                                                >  1:41639/1‑167 (MQ=255)                                                                                                                                                                                               gggggCAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCAACGCGCCCAGTTCAGGCGTGAGATCCGGGAAAGCCTCGTTATCAAAATAGGTCCGCTCGAGACTTGCTCCGTAGATATAAACCATGTTGAg                                                                          <  1:71318/198‑1 (MQ=255)                                                                                                                                                                                                    cAAACAGCGGTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCAGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCAACGCGCCCAGTTCAGGCGTGAGATCCGGGAAAGCCTCGTTATCAAAATAGGTCCGCTCGAGACTTTCGCCGTAGATATAAACCAGGTTGAGTTTCGGGTCAggg                                                             >  1:38119/1‑206 (MQ=255)                                                                                                                                                                                                            ggTATGCCGCACTGAGAAGCCACCATACCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTGTTCAACGCGCCCGGTTCAGGCGTGAGATCCGGGAAAGCCTCGTTATCAAAATAGGTCCGCTCGAGACTTTCGCCGTAGATATAAACCAGGTTGAGTTTCGGGTCAGGGATAGTTTTCGACGGCTCTTTATAATAAGCCGCAAAGTCCGGGTCGCCGTcgcg        <  1:252644/251‑1 (MQ=255)                                                                                                                                                                                                                  ncGCACTGAGAAGCCACCATACCCGCAATCGAGTAATCTATTCCCGGCAGCTGCTGCGTGTGGCTGACATCCAGGCCTTCCTTTTTCAACGCGACCAGTTCAGGCGTGAGATCCGGGCACGCCTCGTTATCAAAATAGGTCCGCTCGAGACTTTCGCCGTAGATATAAACCAGGTTGAGTTTCGGGTCAGGGATAGTTTTCGACGGCTCTTTATAATAAGCCGCAAAGTCCGGGTCGCCGTCGCGTGACTg  >  1:26835/2‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       CGCTTTTCAGCTCTTCTGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTGTGGCTGAAATCCAGGCCTTCATTTTTCAACGCGCCCAGTTCAGGCGTGAGATCCGGGAAAGCCTCGTTATCAAAATAGGTCCGCTCGAGACTTTCGCCGTAGATATAAACCAGGTTGAGTTTCGGGTCAGGGATAGTTTTCGACGGCTCTTTATAATAAGCCGCAAAGTCCGGGTCGCCGTCGCGTGACTG  >  NC_000913/4598571‑4599030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑