Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,577,948	G→A	P4L (CCC→CTC)	mcrC ←	Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,577,948	0	G	A	100.0%	17.7 / NA	9	P4L (CCC→CTC)	mcrC	Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/5);  total (4/5)

GCGTATTGTTTTAGCAAACTCTATTCGCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGCTGTAAAACCCCTTTATTTAATACATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGT  >  NC_000913/4577710‑4578172                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 gcgTATTGTTTTAGCCAACTCTATTCGCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGCTGTAAAACCCCTTTATTTAATAAATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGAGCTGTTCCACt                                                                                                                                                                                                                       >  1:80335/1‑250 (MQ=255)                          ngCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGCTGTACAACCCCTTTATTTAATACATACACACGGATATCAAGAAGATTGTTACAGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAACTGACCCCATGCATCGGAAAGCATGTAATAGATATTACGGACAGGTATCACGAGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                                                                                                             >  1:48972/2‑251 (MQ=255)                                                                                                              tttaATACATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGAGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTc                                                                                                                                                                                       <  1:202497/173‑1 (MQ=255)                                                                                                                         ccccAGGATATCAAGAAGATTGTTACCGGGTATGGCTTAAAGGTTTGCCTGCTTAATTTCCTGTACATAACCCCATGCATAGGTAAGAATGTAATAGATCTTACGGACAGGTATCACGAGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCgg                                                                                              >  1:184599/1‑251 (MQ=255)                                                                                                                                       gaagaTTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCAGGTAATAGATATTACGGACAGGTATCACGAGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGcc                                                                                                           <  1:19113/224‑1 (MQ=255)                                                                                                                                                                                                 ccATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGAGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGAGAGTGNc                      <  1:199752/251‑1 (MQ=255)                                                                                                                                                                                                                     aaTAGATATGACGGACAGGTGTCACGAGCTGTTCCACTATGAGTCCCCGAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCTATGTCCGTCATCACAATTTCATTAAGCCATTGCGGATCCGGAGAGGGGCCATCTTCCAACCCACAGCAGAAGTAGCTATGCCGAATGCGGAAGCCGTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGt  <  1:235974/251‑1 (MQ=255)                                                                                                                                                                                                                               aCGGACAGGTATCACGAGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAACGGGGCGCTATCCGTCATCACAATTTCATTAANCCANNNNNt                                                                                                    >  2:154111/1‑143 (MQ=255)                                                                                                                                                                                                                               aCGGACAGGTATCACGAGCTGTTCCACTATGAGTCCCCTAATAATGTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAAcn                                                                          <  1:154111/169‑2 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 GCGTATTGTTTTAGCAAACTCTATTCGCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGCTGTAAAACCCCTTTATTTAATACATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGT  >  NC_000913/4577710‑4578172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑