Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	38,914	C→T	A68T (GCG→ACG)	caiB ←	gamma‑butyrobetainyl‑CoA:carnitine CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	38,914	0	C	T	100.0%	23.7 / NA	11	A68T (GCG→ACG)	caiB	gamma‑butyrobetainyl‑CoA:carnitine CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

GATAGTGTTATAGGCCGGAAGATTGGTGTACTCCTCGGTGCCGTACTGACCAAAACCGGACAGGTGAGCGATAACCAGTTTCGGGTTGTGCTGCCACAGTACTTCATCGGTAATGCCACGACGGGCAAAGGCCGGACCTTTACTGGCTTCGATGAAGATATCGGTGGTTTCCATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTTGAAAATATTTAACGACAGCGCGTGCAAATTGCGGCGGGAGAGTTGCGGGTAGTTCGGTTGAACGCGAATGGTGT‑CGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGCCAACGG  >  NC_000913/38687‑39085                                                                                                                                                                                                                                    |                                                                                                                                                                             gATAGTGTTATAGGCCGGAAGATTGGTGTACTCCTCGGTGCCGTACTGACCAAAACCGGACAGGTGAGCGATAACCAGTTTCGGGTTGTGCTGCCACAGTACTTCATCGGTAATGCCACGACGGGCAAAGGCCGGACCTTTACTGGCTTCGATGAAGATATCGGTGGTTTCCATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTTGAAAATATTTAACGACAGCATGTGCAAATTGCGGCGGGGGAGtt                                                                                                                                                       >  1:61692/1‑251 (MQ=255)             ggCCGGAANANNNGNNTACTCCTCGGTGCCGTACTGACCAAAACCGGACAGGTGAGCGATNACCAGTTTCGGGTTGNGCNGCCCNAGTNCTTCATCGGTAATGCCACGACGGGCAAAGGCCGGACCTTTACTGGCTTCGATGAAGATATCGGTGGTTTCCATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTNGAANATATTNNANGACAGCNNNTGNNNNNNnngnnnncgnngnnnt                                                                                                                                                     >  2:178389/1‑228 (MQ=255)             ggCCGGAAGATTGGTGTACTCCTCGGTGCCGTACTGACCAAAACCGGACAGGTGAGCGATAACCAGTTTCGGGTTGTGCTGCCACAGTACTTCATCGGTAATGCCACGACGGGCAAAGGCCGGACCTTTACTGGCTTCGATGAAGATATCGGTGGTTTCCATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTTGAAAATATTTAACGACAGCGTGTGc                                                                                                                                                                          <  1:178389/220‑1 (MQ=255)                                      gTGCCGTACTGACCAAAACCGGACAGGTGAGCGATAACCAGTTTCGGGTTGTGCTGCCACAGTACTTCATCGGTAATGCCACGACGGGCAAAGGCCGGACCTTTACTGGCTTCGATGAAGATATCGGTGGTTTCCATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTTGAAAATATTTAACGACAGCGTGTGCAAATTGCGGCggg                                                                                                                                                             >  1:71613/1‑208 (MQ=255)                                                                              gTTTCGGGTTGTGCTGCCACAGTACTTCATCGGTAATGCCACGACGGGCAAAGGCCGGACCTTTACTGGCTTCGATGAAGATATCGGTGGTTTCCATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTTGAAAATATTTAACGACAGCGTGTGCAAATTGCGGCGGGAGAGTTGCGGGt                                                                                                                                                 <  1:184936/180‑1 (MQ=255)                                                                                    ggTTGTGCTGCCACAGTACTTCATCGGTAATGCCACGACGGGCAAAGGCCGGACCTTTACTGGCTTCGATGAAGATATCGGTGGTTTCCATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTTGAAAATATTTAACGACAGCGTGTGCAAATTGCGGCGGGAGAGCTGCGGGGAGTTCGGTTGAACGCGCATGGGGTTCGGCC‑AGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTTCGAAc                                                                   >  1:59607/1‑251 (MQ=255)                                                                                        gtgCTGCCACAGTACTTCATCGGTAATGCCACGACGGGAAAAGGCCGGACCTTTACGGGCTTCGATGAAGATATCGGTGGTTTCCATTAATTTCATAAACGTTTCGCGGCCTTCATCTTTGAAAAGATTTAACGACAGCGTGGTCAAATTGCGGCGGGAGAGTTGCGGGTAGTTCGGTTGAACGCGAATGGTGT‑CGGCCCAGGCGACGTTCTCGATGTAGATAACTTCCGTGCCCCATTCTGCGAACAtt                                                                <  1:83206/250‑1 (MQ=255)                                                                                             gcCACAGTACTTCATCGGTAATGCCACGACGGGCAACGGCCGGACCTTTACTGGCTTCGATGAAGATATCGGTGGTTTCCATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTTGAAAATATTTAACGACAGCGTGTGCAAATTGCGGCGGGagaggtt                                                                                                                                                      >  1:88479/1‑157 (MQ=255)                                                                                                                                            ttACTGGCTTCGATGAAGATATCGGTGGTTTCCATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTTGAAAATATTTAACGACAGCGTGTGCAAATTGCGGCGGGAGAGTTGCGGGTAGTTCGGTTGAACGCGAATGGTGT‑CGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATc            <  1:57673/250‑1 (MQ=255)                                                                                                                                                      cGATGAAGATATCGGTGGTTTCCATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTTGAAAATATTTAACGACAGCGTGTGCAAATTGCGGCGGGAGAGTTGCGGGTAGTTCGGTTGAACGCGAATGGTGT‑CGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGCCAACgn  <  1:69555/250‑2 (MQ=255)                                                                                                                                                                           ccATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTTGACAATATTTAACGACAGCGTGTGCAAATTGCGGCGGGAGCGTTGCGGGTAGGTCGGTTGAACGCGAATGGTGT‑CGGCCCAGGCGACGGTCTCGGTCAAGATAACTTCCGCGCCCCCTTCTGCGAACCTTTGCCCGGCCAAAGGGCCGGCGCTTTCGATAcagggggagac                       >  1:216477/1‑198 (MQ=255)                                                                                                                                                                                          ncGNTTCNCNNCNNGCANCTTTGAAAATATTTAACGACANNGTGTGCAAANTGCGGCGGGAGAGTTGCGGGTAGTTCGGTTGAACGCGAATGGTGT‑CGGCCCAGNCGCCGTTCTNNATNCAGAGNANNNCNNNGCNCCATTCTGCGAACATTTCCNCGNNAAACGGNCCGGCGATTTCGATACCGGAGAAGAc                       <  2:59607/192‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                             GATAGTGTTATAGGCCGGAAGATTGGTGTACTCCTCGGTGCCGTACTGACCAAAACCGGACAGGTGAGCGATAACCAGTTTCGGGTTGTGCTGCCACAGTACTTCATCGGTAATGCCACGACGGGCAAAGGCCGGACCTTTACTGGCTTCGATGAAGATATCGGTGGTTTCCATTAATTTCAGAAACGCTTCGCGGCCTTCATCTTTGAAAATATTTAACGACAGCGCGTGCAAATTGCGGCGGGAGAGTTGCGGGTAGTTCGGTTGAACGCGAATGGTGT‑CGGCCCAGGCGACGTTCTCGATCCAGATAACTTCCGCGCCCCATTCTGCGAACATTTGCCCGGCAAACGGTCCGGCGATTTCGATACCGGAGAAGACAACGCGCAATCCGGCCAACGG  >  NC_000913/38687‑39085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑