Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	250,938	C→T	A14V (GCC→GTC)	dinB →	DNA polymerase IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	250,938	0	C	T	100.0%	26.1 / NA	10	A14V (GCC→GTC)	dinB	DNA polymerase IV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/5);  total (5/5)

CTGGATAAGCAGCAGGTGCTTTCGCAGCGAACGCGTTAAATGCTGAATCTTTACGCATTTCTCAAACCCTGAAATCACTGTATACTTTACCAGTGTTGAGAGGTGAGCAATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGCCGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGATATCCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCACATCTCACCTTGCTTCCGGGGCGCTTTGACGCCTACAAAGAAGCCTCAAATCATATCCGTGAAATCTTCTCGCGCTACACC  >  NC_000913/250789‑251179                                                                                                                                                      |                                                                                                                                                                                                                                                  cTGGATAAGCAGCAGGTGCTTTCGCAGCGAACGCGTTAAATGCTGAATCTTTACGCATTTCTCAAACCCTGAAATCACTGTATACTTTACCAGTGTTGAGAGGTGAGCAATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGTCGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGATATCCCTATTGCTAGTGGCGGCAGCCGCGAACGTCGGGGTGTGATCAgn                                                                                                                                                              <  1:77544/235‑2 (MQ=255)        ngcagcaGGTGCTTTCGCAGCGAACGCGTTAAATGCTGAATCTTTACGCATTTCTCAAACCCTGAAATCACTGTATACTTTACCAGTGTTGAGAGGTGAGCAATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGTCGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGATATCCcta                                                                                                                                                                                                       >  1:154621/2‑187 (MQ=255)                         cAGCGAACGCGTTAAATGCTGAATCTTTACGCATTTCTCAAACCCTGAAATCCCTCTATACTTTACCAGTGTTGAGAGGTGAGCAATGCGTCAAATCATTCATGTGGATATGGACTGCTTTTTCGTAGCAGTGGCGATGCGCGACAATCCCGCCCTGCGCGATATCCCTATTGCTATTGGCGGCAGCCGCGACCGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTCCGTAgcg                                                                                                                      >  1:177074/1‑251 (MQ=255)                                cgcgTTAAATGCTGAATCTTTACGCATTTCTCAAACCCTGAAATCACTGTATACTTTACCAGTGTTGAGAGGTGAGCAATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGTCGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGATATCCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTAGCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGcc                                                                                                               <  1:87659/251‑1 (MQ=255)                                      aaaTGCTGAATCTTTACGCATTTCTCAAACCCTGAAATCACTGTATACTTTACCAGTGTTGAGAGGTGAGCAATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGTCGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGATATCCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAgg                                                                                                         <  1:71916/251‑1 (MQ=255)                                                 nnttNNNNNTTTNTCAAACCCTGAAATCACTGTATACTTTACCAGTGTTGAGAGGTGAGCAATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGTCGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGATATCCcta                                                                                                                                                                                                       <  2:154621/144‑1 (MQ=255)                                                                                          ccAGTGTTGAGAGGTGAGCAATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGTTGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGATATCCCTATTGCTATTGGCGGCAGCAGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCACATCTCACCTTGCTTCCGGGGCGCTTTGAc                                                     >  1:56079/1‑251 (MQ=255)                                                                                               gtTGAGAGGTGAGCAATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGTCGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGATATCGCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATTGCGCTCAAATTATGCCCACATCTCACCTTGCTTCCGGGGCGCTTTGGCGCCTa                                                <  1:104788/251‑1 (MQ=255)                                                                                                                                         naCTGCTTTTTCGTAGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGATATCCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCACATCTCACCTTGCTTCCGGGGCGCTTTGACGc                                                   >  1:69649/2‑206 (MQ=255)                                                                                                                                             gCTTTTTCGTCGCAGCGGAGATGCGCGACAATCCCGCCCTGCGCGATATCCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATTGCGCCCAAATTATGCCCACATCTCACCTTGCTTCCGGGGCGCTTTGACGCCTACACAGAAGCCTCAAATCATATCCGTGAAATCTTCTCGCGCTACAcc  >  1:6758/1‑251 (MQ=255)                                                                                                                                                      |                                                                                                                                                                                                                                                  CTGGATAAGCAGCAGGTGCTTTCGCAGCGAACGCGTTAAATGCTGAATCTTTACGCATTTCTCAAACCCTGAAATCACTGTATACTTTACCAGTGTTGAGAGGTGAGCAATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGCCGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGATATCCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCACATCTCACCTTGCTTCCGGGGCGCTTTGACGCCTACAAAGAAGCCTCAAATCATATCCGTGAAATCTTCTCGCGCTACACC  >  NC_000913/250789‑251179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑