Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,450,847	G→A	T173I (ACC→ATC)	rplB ←	50S ribosomal subunit protein L2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,450,847	0	G	A	92.3%	27.9 / NA	14	T173I (ACC→ATC)	rplB	50S ribosomal subunit protein L2
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (7/5);  minor base C (1/0);  total (8/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

TACCAAAGTTACGACCTTCACCACCACCATGTGGGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGGTGACATAAGCACCATCACGAGCAACGATCTGAACGTAAGTACCAGCGGAACGTGCCAGCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGATGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCG  >  NC_000913/3450641‑3451092                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      tACCAAAGTTACGACCTTCACCACCACCATGTGGGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACCCCACGCCAGCGTGCAGCACCTGCTTTCCCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGTATTCTTCTTCTACTTTACGCATTTCACCAGAACGTAGACGCAGGATGACATAAGCCCCATCACGAGCAACGATCTGAACGTAAGTAc                                                                                                                                                                                                             <  1:105681/249‑1 (MQ=255)                   tgccaccaccaTGTGGGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGATGACATAAGCACCATCACGAGCAACGATCTGAACGTAAGTACCAGCGTAACGTGCCAGCTg                                                                                                                                                                                          <  1:74323/248‑1 (MQ=255)                                                   aTCGCGGTACCCCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTTCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTGCTTCTACTTTACGCATTGCACCAGAACGCAGACTCAGGGTGACATAAGCACCATCACGAGCAACGATCTGAACGTAAGTACCAGCGGAACg                                                                                                                                                                                                   <  1:125332/209‑1 (MQ=255)                                                            ncgcgAACGGTCGGACGAACACCACGCCAGCGTGCAGCACTTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGATGACATAAGCACCATCACGAGCAACGATCTGAACGTAAGTACCAGCGGAACGTGCCAGCTGAc                                                                                                                                                                                        >  1:63795/2‑211 (MQ=255)                                                                                      ccAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGATGACATAAGCACCATCACGAGCAACGATCTGAACGTAAGTACCAGCGGAACGTGCCAGCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAg                                                                                                                                                    >  1:58557/1‑221 (MQ=255)                                                                                           gTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGATGACATAAGCACCATCNCg                                                                                                                                                                                                                                    >  2:153965/1‑136 (MQ=255)                                                                                           gTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGATGACATAAGCACCATCAcn                                                                                                                                                                                                                                    <  1:153965/136‑2 (MQ=255)                                                                                                gcaCCTGCGTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCTCGGCAGTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGATGACATAAGCACCATCACGAGCAACGATCTGAACGTAAGTACCAGCGGAACGTGCCAGCGGACCGCCTTTACCTGTTTTCATTTc                                                                                                                                                                  <  1:208183/197‑1 (MQ=255)                                                                                                                   cangcancnNANNCTCNGCATTGCCAACTTCGCCCAGANNTGCACGGCANTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGATGACATAAGCNCCAGCACGANNAANGATCGNANNNTNNNTANCAGCGGAACGTGCCAGCTGNCCNNCTTTACNTGGTTTCATTTCTACGTTATGAACAg                                                                                                                                                    <  2:58557/189‑1 (MQ=255)                                                                                                                    gcgcAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGATGACATAAGCACAATCACGAGCAACGATCTGACCGTAAGTACCAGCGGAACGTGCCAGCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCa                                                                                         >  1:120493/1‑250 (MQ=255)                                                                                                                                                  gCCCAGAGTTGCACGGCAGTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGCTGCCATAAGCACCATCACGAGCAACGCTCTGAACGTAAGTACCAGCGGAACGTGCCAGCt                                                                                                                                                                                           >  1:253178/1‑122 (MQ=255)                                                                                                                                                             cACGGCAGTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGATGACATAAGCACCATCACGAGCAACGATCTGAACGTAAGTACCAGCGGAACGTGCCAGCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGt                                                                                                                                                   >  1:9782/1‑151 (MQ=255)                                                                                                                                                                                 ttACGCATTTCACCAGAACGCAGACGCAGGATGACATAAGCACCATCACGACCAACGATCTGAACGTAAGTACCAGCGGAAAGTGCCAGCTGACCGCCTTTACCTGGTTTAATTTCTACGTTCTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTc                                                           >  1:101293/1‑219 (MQ=255)                                                                                                                                                                                                          gcagGATGACATAAGCACCATCACGAGCACCGATCTGAACGTAAGTACCAGCGGAACGTGCCACCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGCTTGATTGAAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGATGTAACGGCGTTCACCGTCTTTGTACAGAACCAGcgcg  >  1:245529/1‑251 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      TACCAAAGTTACGACCTTCACCACCACCATGTGGGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTGCTTCTACTTTACGCATTTCACCAGAACGCAGACGCAGGGTGACATAAGCACCATCACGAGCAACGATCTGAACGTAAGTACCAGCGGAACGTGCCAGCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGATGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCG  >  NC_000913/3450641‑3451092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑