Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,084,483	G→A	L477L (CTG→TTG)	speA ←	biosynthetic arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,084,483	0	G	A	93.3%	28.8 / ‑3.0	15	L477L (CTG→TTG)	speA	biosynthetic arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (7/7);  total (8/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01

GCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGCAGAGAACGGCGAGTTCCCGGTTC  >  NC_000913/3084314‑3084684                                                                                                                                                                          |                                                                                                                                                                                                          gCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACAGTTTGTCCGCCATACGTTCCTGCAACGCGTCGATAATCGGACGATGAGCACGGTTTTGCGGATCCAGCTGCTTTTg                                                                                                                                                        <  1:165284/221‑1 (MQ=255)                          ccAGCAGCACAGCGCGACGTTCCGGCACTTGCTCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAACTCGTCGATAATCGGACGATGAGCACGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAgg                                                                                                 >  1:89364/1‑251 (MQ=255)                                gcaCAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTACGGCATCGACTGGAACAGCGCGAAGTTGCCGTCAATTTTGTCCGACATCCGTTCCTGCAACTCGTCGATAATCGGACGATGAGCACgg                                                                                                                                                                               >  1:180199/1‑167 (MQ=255)                                     ncgcgACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTTTTGCGGATc                                                                                                                                                                    >  1:67695/2‑173 (MQ=255)                                                        naTCCAGCACTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCAGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAACTCGTCGATAATCg                                                                                                                                                                                             >  1:69358/2‑129 (MQ=255)                                                            cAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGGCGATCCCCCATGCGTCCGGCATCGACTTGAACAGCGAGAAGTTTACGTACATTTTGTCCGCCATACGTTCCTGCAACTCGTCGATAATCggcc                                                                                                                                                                                          <  1:229846/128‑3 (MQ=255)                                                                          gCAGAACCGGGAACAACTGGTCGATCCCCCATGCCTCCGGCATCGACTGGAACCGCGAGAAGTTGACGTACCTTATGTTCGCCATACGTTCCTGCAACTCGTCGATAATCGGACGATGAGCACGGTTTTGCGGACCCacc                                                                                                                                                                >  1:109514/1‑138 (MQ=255)                                                                                ccGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAACTCGTCGATAATCGGACGATGAGCACGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGACATGAATGTCGTGCAGAGCCATCTGACTg                                           >  1:177081/1‑251 (MQ=255)                                                                                         acttgTCGATCCCCCATGCGTTCGGCATCGACTGGAACAGCGAGAAGTTGACGGTCATTTTGTCCGCCATACGTTCCTGCAACTCGTCGATAATCGGACGATGAGCACGGTTTTGCg                                                                                                                                                                        <  1:188248/113‑1 (MQ=255)                                                                                         acTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAACTCGTCGATAATCGGACGATGAGCACGGTTTTGCGGATCCAGCTGCTTGTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAg                                                        <  1:117963/229‑1 (MQ=255)                                                                                         acTGGNNGANNNCCNNTGCGTCCGGCATCGACTGGAACANCGAGAAGTTGACGTACATTTTGTACGCCANANGNTCCTNNAACNCGTCGATAATNGGACGATGAGCACGGTTTTGCg                                                                                                                                                                        >  2:188248/1‑117 (MQ=255)                                                                                                                                         tGACGTACATTTTGTCCGCCATACGTTCCTGCAACTCCTCGATAATCGGACGATGAGCACGGTTTTGCGGCTCCAGCTGCTTTTGCACTTCATGGAACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGCAGCGAACGGCg              >  1:96698/1‑223 (MQ=255)                                                                                                                                                     nnnccGNCATACGTTCCGGCAACTCGTCGATAATCGGACGATGAGCACGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGTCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCg                                                                                     <  2:154697/137‑1 (MQ=255)                                                                                                                                                                  ttCCTTCAACTCGGCGATAATCGGACGATGAGCACTGTGGTTCCGAGCCAGCTGCTTTTTCACTTCATGTTACATGCTCAAATAAAGCTGCTCAGCCCATg                                                                                                               <  1:224310/101‑1 (MQ=255)                                                                                                                                                                    ccTGCAACTCGTCGATAATCGGACGATGAGCACGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGGCAGATGCTCAAATAAAGCGTCTCAGCCCATGCACGGTCTGGCAGGCTAAAGATGCCGGAAGATTAGCCGATATGAATGGGTTGGAGATTCATCTGACTGTCGTGTAACCATTCACGCAGAGAACGGCGAGTTCCCGGTTc  <  1:142824/208‑1 (MQ=255)                                                                                                                                                                          |                                                                                                                                                                                                          GCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGCAGAGAACGGCGAGTTCCCGGTTC  >  NC_000913/3084314‑3084684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑