Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,615,442	G→A	R34R (CGC→CGT)	yjjW ←	putative glycyl‑radical enzyme activating enzyme YjjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,615,442	0	G	A	100.0%	25.6 / NA	12	R34R (CGC→CGT)	yjjW	putative glycyl‑radical enzyme activating enzyme YjjW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/4);  total (8/4)

CCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGAATACGAGTGTTGCGTGCGGCTTCT  >  NC_000913/4615200‑4615596                                                                                                                                                                                                                                                   |                                                                                                                                                           ccTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGACATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGATCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAACGCAGAtt                                                                                                                                                    >  1:231219/1‑251 (MQ=255)                  tGCTTTGCGGACATGGCTAAGCACGTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAACGCAGATTGCAGCCCTGCAAAAAc                                                                                                                                    <  1:4657/250‑1 (MQ=255)                   gCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTGTGCCGTCAACAATCTGCATCGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTGCACGGATTGTGACAGTTTTTGCAACGCAGATTGCAGCCCTGCAAAAAc                                                                                                                                    <  1:128183/249‑1 (MQ=255)                                                                       gCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAACGCAGATTGCAGCCCGGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTg                                                                              <  1:239596/251‑1 (MQ=255)                                                                                      aacacCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAACGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATctg                                                               >  1:61928/2‑251 (MQ=255)                                                                                                                  cTCGCAAACCACAGCGTTCCACACCACTTTACCGTCAGCAATCTGCAACCCCTGCTGAGGACACTGTCGCACGCAATCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAACGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCACCGCCGGAGAAGGGGATAATATTACTGACTAAAACGCATCTGCTGTTCATGGCTTATCACGCACGGCTgg                                   >  1:99289/1‑251 (MQ=255)                                                                                                                      cAAACCACAGCGTTCCACACAACTTTGCCGTCAACAATCTGAAACGCCTGATGCGGACACTGTGGGACGCACTCCCCCCACTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGCTTTTGCAACGCCGATTGCAGCCCTGCCAAACCAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATgg                                                     >  1:144337/1‑229 (MQ=255)                                                                                                                                                        caaTCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAACGCAGATTGCAGCCCTGCAAAAACAGAGcc                                                                                                                              <  1:12544/122‑1 (MQ=255)                                                                                                                                                                                      ncacgcacTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAACGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGAATACGAGTGTTGCGTGCGGcttct  >  1:67574/2‑216 (MQ=255)                                                                                                                                                                                         cgcacTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTCTTGCAACGCAGATTGCAGCCCTGCAAAAACAGAGACAGACGACTGCCTGGCCCGTCAACgaag                                                                                                   >  1:167043/1‑113 (MQ=255)                                                                                                                                                                                               ccccACAGTCATTGCCACGTCCCATCGTCCCCGGATTGTGACAGTTTTTGCAACGCAGATTGCAGCCCTGCAAAAACagcg                                                                                                                                >  1:133883/1‑79 (MQ=255)                                                                                                                                                                                                                     cATCGTCCACGGATTGTGACAGTTTTTGCAACGCAGATTGCAGcc                                                                                                                                              >  1:82116/1‑45 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                           CCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGAATACGAGTGTTGCGTGCGGCTTCT  >  NC_000913/4615200‑4615596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑