Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,854,523	C→T	Y62Y (TAC→TAT)	fhlA →	DNA‑binding transcriptional activator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,854,523	0	C	T	100.0%	19.9 / NA	9	Y62Y (TAC→TAT)	fhlA	DNA‑binding transcriptional activator FhlA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

CCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCC  >  NC_000913/2854350‑2854768                                                                                                                                                                              |                                                                                                                                                                                                                                                      ncGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTATGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACgcagca                                                                                                                                                                              >  1:30277/2‑247 (MQ=255)          gATCTCGGACAACAAGGGTTGTTCTACATCACTCGGACACTATTGCCGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCGATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTATGCGTCGCGTGAAAAAGACCCCCCCATTAAAGATGAAGACGAATCTCTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCgcgcn                                                                                                                                                                  <  1:49204/250‑2 (MQ=255)               cGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGACGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTATGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGAACGCAGCATTTTGTCGCGCCCTGAt                                                                                                                                                             >  1:182702/1‑250 (MQ=255)                                                            gcCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTATGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCATCATTTTGTCGCTCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGc                                                                                                               <  1:85391/251‑1 (MQ=255)                                                                gATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTATGCGTCGCGTGCAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTcg                                                                                                                                                                      >  1:205839/1‑192 (MQ=255)                                                                                                    gTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTATGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTg                                                                       <  1:98055/251‑1 (MQ=255)                                                                                                           gTTCTGCGCTCGCCGACAACGCGGCTCTTGTGTTGTGGGAAGCGCAGACTCAAGGTGCGTCTTATTCTGCCTCGCGTGAAAAAGACACCACCCTTAAATATGAAGTCGAAACTGTTCTGGCACCCGGTCCGGTACGCAGCCTTTTGTCGACCCCTGATCCGCTGCATTGCAGTTACGCAGAATTTTGTGCAACCTGGCCGCCGCTGGACGCAGGTGGACTATACCCAAAATTTGGTCACTATTGCCTGATg                                                                >  1:232875/1‑251 (MQ=255)                                                                                                                                                gCAAGCGCAGACTCAACGTGCGTCTTATTATGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCtgtg                           >  1:12669/1‑251 (MQ=255)                                                                                                                                                                          attaTGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGTCGCAGGTGGGCTATTCCCAAAATTTGGTCACGATTGCCTGATGCCAGTGGCGGCGTAAGGGCATATTGTTGGTGGCTGTGAATTTATTCGTTATGACGATCGccc  <  1:98778/250‑1 (MQ=255)                                                                                                                                                                              |                                                                                                                                                                                                                                                      CCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCC  >  NC_000913/2854350‑2854768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑