Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,906,661	C→T	E427E (GAG→GAA)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,906,661	0	C	T	100.0%	33.4 / NA	13	E427E (GAG→GAA)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/9);  total (4/9)

GAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTT  >  NC_000913/2906477‑2906820                                                                                                                                                                                         |                                                                                                                                                                gAAGTGATCATTCAGGCAGCGGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAACGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCGTAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCATATAAAGTCAGTCTTATGCCGGGCCTTTGATTTCTTTGCGTCCGGTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTAt                                                                                               <  1:127426/251‑1 (MQ=255)                             cGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTGCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAAGGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATTTCTTTACGACCGTTGTACGGTg                                                                                                                                           <  1:120772/179‑1 (MQ=255)                                                 gcggATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGAGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATTTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATGTAGCAac                                                                                       <  1:85568/211‑1 (MQ=255)                                                                  ggCGGTTCTTCTGGAGGTGGCGGCGCACCCGGCCGCTTGTAACTCAACCATAAAAAATGCCAGCCCGGATGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATTTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTAc                                     <  1:22694/244‑1 (MQ=255)                                                                   gCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTCACTCAACCATAAAAAATGCAAGGCCGGGGGATGGCCTTTTTAAATCAGATAAAGCAACACTTATGCCTGGGCTTTGATTTCTTTACCACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATTCGAATCAGCTGGTTGTATTTAGCAACACGGACAGACCGGCTCCTTGAACCAGTTTTGATCTGGCCTGCAGCCGTACCAAAAGcc                             >  1:97559/1‑251 (MQ=255)                                                                                                     cTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATTTCTTTACGACCGTTGTACGGTGCTTTTTCg                                                                                                                                   <  1:40901/115‑1 (MQ=255)                                                                                                       ggTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGACCAAGTCAGTCTTATGCCTGGCATTTGATTTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTCAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCATGCAGCAGTACCAACAg                               >  1:100141/1‑213 (MQ=255)                                                                                                        gTAACTCAACCATAAAAACTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATTTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCttcttc                                                                                                                     >  1:202525/1‑126 (MQ=255)                                                                                                                     aaaaaaTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATTTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTggn                                                 <  1:68922/181‑2 (MQ=255)                                                                                                                                                             aaaGTCAGTCTTATGCCTGGCCTTTGATTTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTgcagn                                          <  1:204701/148‑2 (MQ=255)                                                                                                                                                                               ggCCTTTGATTTCTTTACGACCGTGGTACGGTGCTTTTCCGCCCAGAGCTTCGTCGAGACGAATCAGCGGGTGGTAGTGAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGtt  <  1:244794/170‑1 (MQ=255)                                                                                                                                                                                 ccTTTGATTTCTTTACGACCGTTGTACGGTGCGTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGtn                                                                                 <  1:33337/89‑2 (MQ=255)                                                                                                                                                                                  cTTTGATTTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCCGCTGGTTGGATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGttt                                                          >  1:16231/1‑111 (MQ=255)                                                                                                                                                                                         |                                                                                                                                                                GAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTT  >  NC_000913/2906477‑2906820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑