Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	618,222	C→T	intergenic (+184/‑32)	entF → / → fepE	apo‑serine activating enzyme/polysaccharide co‑polymerase family protein FepE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	618,222	0	C	T	100.0%	20.4 / NA	10	intergenic (+184/‑32)	entF/fepE	apo‑serine activating enzyme/polysaccharide co‑polymerase family protein FepE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/7);  total (3/7)

AAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGG  >  NC_000913/618037‑618386                                                                                                                                                                                          |                                                                                                                                                                     aaaTTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATTTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGGGAGCGCCGTGGGGAGTTCTCCCTTTTGGTCTCATTTTCAGGTTATACGCCATGTCATCACTGAATATTAAACAGGGAAGTGACg                                                                                                     <  1:161315/251‑1 (MQ=255)                           aTTACAGAAAATAATTATGGGTTTTTTATTTGTTTAATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCTCCCTATTGGACTCATTTTCATGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCg                                                                           <  1:126890/251‑1 (MQ=255)                                aGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCTCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACt                                                                                                                            <  1:208980/197‑1 (MQ=255)                                                                            atgaATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCTCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGc                                                                            <  1:137310/201‑1 (MQ=255)                                                                                                gagTGAATGTTGCATTCAGTGTAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCTCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTGTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGttt                             <  1:96151/228‑1 (MQ=255)                                                                                                  ntGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCTCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACg                                                                                                     >  1:76100/2‑154 (MQ=255)                                                                                                                             ttACAAACTTCAGCTTCGGATAATTTTCACCAACTGGTAAAGTGAGCGGAATGGAGTTTTTTACTTATTGGACTCATTTTTAGGCTATACCCCATGTCATAACTGACTCTTAAACACGGAAGAGAAGCTCATTTTCCCgc                                                                                        >  1:231686/1‑139 (MQ=255)                                                                                                                                         gCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCTCCCTATTTGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATTTTAAACAGGGAAGTGACGCTCATTTTCCCGAGTATCCTCTTGCTGCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATgg  <  1:172086/214‑1 (MQ=255)                                                                                                                                          cTTCGGATAATTATCACCAACTGGGAAAGTGAGCGCCGTGGAGATTTCTCCCTATTgn                                                                                                                                                             <  1:31293/58‑2 (MQ=255)                                                                                                                                          cTTCGAATNATTANCNNNAACTGNTAAAGTGAGNGCCGTGGAGATTTCTCCCTNTTgg                                                                                                                                                             >  2:31293/1‑58 (MQ=255)                                                                                                                                                                                          |                                                                                                                                                                     AAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGG  >  NC_000913/618037‑618386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑