Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,769	C→T	P460L (CCT→CTT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,769	0	C	T	100.0%	30.0 / NA	12	P460L (CCT→CTT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

CGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGT  >  NC_000913/23536‑23988                                                                                                                                                                                                                                          |                                                                                                                                                                                                                            cgtcgtTGCGCTGCTGCAGGAAAAAGGCGCGCTTCTGCACGTTGAGAAAATGCAGCGCAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCTTGACTGGTGTATCTcc                                                                                                                                                                                                             <  1:1805/250‑1 (MQ=255)                              cgcTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCTTGACTGGTGTATCTCCCGTCAGCGCan                                                                                                                                                                                                  <  1:66694/232‑2 (MQ=255)                                                               aTCCGTGCTGCTGGCGTCACAAAACGCCGATCAGCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTTAAAGAGAGCAAAGGCGTTCAGTGGATCCCGGGCTGGGGCCAGGTGCGTATCGAGTCGATGGTTGCTAACCGTCTTGACTGgtg                                                                                                                                                                                                                    <  1:220777/181‑1 (MQ=255)                                                                                 gcaAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCTTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTgn                                                                                                                                                                     <  1:160293/209‑2 (MQ=255)                                                                                       cGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCTTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATc                                                                                                                                             <  1:61083/228‑1 (MQ=255)                                                                                                     cgcgcgACGCCGCAGTGGTTCGTCAGCATGGATCAGAACGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCCGTGGATCCCGGACTGGGGCCAGGCGAGTATCGAGTCGATGGTTGCTAACCGTCTTGACTGGTGTATCTCCCGTCAGCGCa                                                                                                                                                                                                   >  1:183371/1‑160 (MQ=255)                                                                                                                           tCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAACGGCGTGCAGTGGATCCCGGACTGGGGCCCGGCGCGTATCGAGTCGATGGTTGCTAACCGTCTTGACTGGTGTATCTCCCGTCAgag                                                                                                                                                                                                     >  1:125473/1‑134 (MQ=255)                                                                                                                            cAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCTTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAg                                                                                  <  1:147883/250‑1 (MQ=255)                                                                                                                                                                gAAAGAGATCAAAGGCGTGNAGTGGATCCCGGACTGGGGCCAGGCGCGTATNGNGTCGATGGTTGCTAACCGTCTTGACTGGTGTATCTCCCGTCAGCGCACNTGGGGTGTACNGATNNCACTNTNCNTGCACAAAGACACGGAAGAGCTGCATCCGNGTACCCTTGAACTGATGGAAGAANNGGNNNAANNCGTTg                                                                                                   <  2:189493/197‑1 (MQ=255)                                                                                                                                                                gAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCTTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTg                                                                                                   >  1:189493/1‑197 (MQ=255)                                                                                                                                                                     agaTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTATTGCCTGGTGTATCTCACGTCAGCACAACTGTGGTGTACAGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGAAACCGCGTACCCTTGAAATGATCGAAGAAGTGGCAAACCGCGTTGAAGTCGATGGCATCCAGGCGTGGTAGGATCTCAGTGCGAAAGAGATCCTCGGcgacga                                         >  1:129580/1‑250 (MQ=255)                                                                                                                                                                                                            cgcgTATCGAGTCGATGGTTGCTAACCGTCTTGACTGGCATATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGCTGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATCGGATGt  >  1:38282/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                            CGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGT  >  NC_000913/23536‑23988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑