Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	111,101	A→G	I20V (ATA→GTA)	mutT →	8‑oxo‑dGTP diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	111,101	0	A	G	100.0%	24.8 / NA	9	I20V (ATA→GTA)	mutT	8‑oxo‑dGTP diphosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/5);  total (4/5)

AGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACAGGCGGTGGTGCGTGAACTTCAGGAAGAAGTCGGGATTACCCCCCAACATTTTTCGCT‑ATTTGAAAAACTGGAATATGAATTCCCGGACAGGCAT‑ATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCG  >  NC_000913/110857‑111325                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   aGGATGACGGCTCTGCATCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGGCGTAACGATCCTTGTCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGGAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTGTAAcgc                                                                                                                                                                                                                              <  1:190187/251‑1 (MQ=255)                                                                                                                             aTAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGAGACTTTTTTCTAGGAATAAGACAATGAAAAAGCTGCAAATTGCGGTAGGGATTATTAGCAACGAGAACAATGCAATATTTGTAACGAGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTca                                                                                                                                                                                   >  1:36582/1‑169 (MQ=255)                                                                                                                                                         ncAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTGTAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACAGGCGGGGGTGCGTGAACTTCAGGAAGAAATGGGGATTACCCCCCAACATTTTTCGCT‑ATTTGACAAACTGGa                                                                      >  1:160232/2‑250 (MQ=255)                                                                                                                                                            ggATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTGTAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACAGGCGGTGGTGCGTGAACTTCAGGAAGAAGTCggg                                                                                                             <  1:14127/209‑1 (MQ=255)                                                                                                                                                                                                       gCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTGTAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACAGGGGGTGGTGCGTGAACTTCAGGAAGAAGGCGGTATTACCCCGCAACATTTTTCGCT‑ATTTGAAAAACTGGAATATGAAGTCCCGGACAGGCAT‑ATAACACTGTGGTTTTGGCTGGTCg                      <  1:143900/251‑1 (MQ=255)                                                                                                                                                                                                       gCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTGTAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACAGGCGGTGGTGCGTGAACTTCAGGAAGAAGTCGGGATTACCCCCCAACATTTTTCGCT‑ATTTGAAAAACTGGAATATGAATTCCCGGACAGGCAT‑ATAACACTGTGGTTTTGGCTGGTc                       <  1:202671/250‑1 (MQ=255)                                                                                                                                                                                                                ggtaggtaTTATTCGCAACGAGAACAATGAAATCTTTGTAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACAGGCGGGGGGGCGGGAACTTCAGGAAGAAGTCGGGATTACCCCCCAACATTTTTCGCT‑ATTTGAAAAAACGGAATAAGAATTCCCGGACAAGCAT‑ATAACACTGCGGTTTTGGCTGGGCGaaaggtggg             >  1:20685/1‑244 (MQ=255)                                                                                                                                                                                                                      tattatGCGCAACGAGAACAATGAAATCTTTGTAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACAGGCGGTTGTGCGTGAACTTCAGGAAGAAGTCGGGATTACCCCCCAAc                                                                                                <  1:114343/164‑1 (MQ=255)                                                                                                                                                                                                                           ttCGCAACGAGAACAATGAAATCTTTGTAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACAGGAGTTTCCCGGCGGCACAATTGAAATGGGTGAAACGCCGGCACAGGCGGTGGAGAGAGGACCTCAGGGACAAGTCGGGGTTCACCCCCAACATTTTTCGCTTATTT‑AAAAAATGGAATATGAATTTCCGGACCAGCATTATAA‑ACTGTGGGTTTGGCTGGTTGAAAGCTTGGAAGGGGAggcg  >  1:118146/1‑248 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   AGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACAGGCGGTGGTGCGTGAACTTCAGGAAGAAGTCGGGATTACCCCCCAACATTTTTCGCT‑ATTTGAAAAACTGGAATATGAATTCCCGGACAGGCAT‑ATAACACTGTGGTTTTGGCTGGTCGAACGCTGGGAAGGGGAGCCG  >  NC_000913/110857‑111325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑