Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	788,642	C→T	A66T (GCC→ACC)	galM ←	galactose‑1‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	788,642	0	C	T	100.0%	16.2 / NA	8	A66T (GCC→ACC)	galM	galactose‑1‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/4);  total (4/4)

ACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTA  >  NC_000913/788418‑788861                                                                                                                                                                                                                                 |                                                                                                                                                                                                                            aCCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTAGGCGAAAGCTGCACGGTTTCACCGTCAAAGTTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGTCTGATCCTGATAGCATTCCGGGCTgg                                                                                                                                                                                                   <  1:180793/251‑1 (MQ=255)                    ccATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGTCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTc                                                                                                                                                                                >  1:19763/1‑251 (MQ=255)                                     ggCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGACCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCCCGGTTTCACCGTCAAAGGTATAACGGCTATTGGCAATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGTCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCgg                                                                                                                                                               >  1:121386/1‑251 (MQ=255)                                                   gACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAGCCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGTCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATan                                                                                                                                                   <  1:156568/249‑2 (MQ=255)                                                                                   cGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGAGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGTCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATc                                                                                                                   >  1:107982/1‑249 (MQ=255)                                                                                                                                   ctgggCGAAAGCGTCACGGTGTCACCGTCAAAGGTAGAACGGCTCTTGGCGCTACGGTTGGCATCACGACCAATAGAGGCCCCCAGAAACGCGGTCTGATGCTGATAGCATTCCGGGCTGGCACACCCGAGCCNCGCNNNNNggnn                                                                                                                                                                          >  2:160575/4‑144 (MQ=255)                                                                                                                                                  aCGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTGGGCATAACGACCAAGAGAGGCCCCCAGAAACGCGGTCTGATCGTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCTCCCCGGTCCATTAGCGGTACGTCCAGCCCTGCGTTGTTACGCAAAGTTATCAGTCGGTACGGCTGACCATCGGGTGc                                                  <  1:167852/251‑1 (MQ=255)                                                                                                                                                                                                   aaCGACCAATAGAGGCCCCCAGAAACGCGGTCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGGCGGTACGGCTGCCCATCGGGTGCCAGTGCGGGAGTGTCGTGCAGCACTGTCCTGCTCCTTGTGATGGTTTa  <  1:100880/250‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                            ACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTA  >  NC_000913/788418‑788861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑