Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,009,970	G→A	E403K (GAG→AAG)	ygeY →	putative peptidase YgeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,009,970	0	G	A	100.0%	20.1 / NA	9	E403K (GAG→AAG)	ygeY	putative peptidase YgeY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/7);  total (2/7)

CTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTCACTTATTCCCCTCCGGTTCGCCGGAGGGTTTTTGGAGTTTGCTATGCGCGTATTGATCAAAAACGGCACTGTCGTTAACGCAGATGGACAAGCCAAACAGGATTTGCTGATTGAAAGCGGGATTGTTCGCCAGTTGGGCAACAATATTTCGCCGCAGCTCCCGTATGAAGAAATTGATGCCACTGGCTGTTACGTTTTCCCTGGCGGCGTGGATGTCCATACGCATT  >  NC_000913/3009804‑3010211                                                                                                                                                                       |                                                                                                                                                                                                                                                  ctcAACTAACGGCGTATCTATCATGGGCCGTCACGGCAGTCCGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTTCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAAAGTAATTACTCTTCACGTATTCCCCTCCGTTTCGCCGGAGGGTTTGTGGAg                                                                                                                                                                                                <  1:193618/218‑1 (MQ=255)                                        tCCGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAAAGTAATTACTCTTCACTTATTCCCCTCCGGTTCGCCGGAGGGTTTTTGGAGTTTGCTATGCGCGTATTGATCAAAAACGGCACTGTCGTTAACGCAGATGGACAAGCCAAACAGGATTTGCt                                                                                                                         <  1:210275/250‑1 (MQ=255)                                                             gggTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCGGGCAACAAAGTAATTACTCTTCACTTATTCCCCTCCGGTTCGCCGGAGGGTTTTTGGAGTTTGCCATGCGCGTATTGATCAAAAACGGCACTGTCGTTAACGCAGATGGACAAGCCAAACAGGATTTGCTGATTGAAAGCGGGCTTGTTCGc                                                                                                   <  1:169327/251‑1 (MQ=255)                                                                                                         aaactctccTGGGGACCTGTGCTGTGATGTACGCTGCAATCCCGTTAAGCTGGCTTGCAACAAAGTAATTACTCGTCTCTTATTCCCCTCCGGTTCGCCGGATGGTTTTGGGAGTGTGGTATGCGCGTATTGATCTAAAACGGTACGGTCGTTAACTCATATGGACAAGCCAAACAGGATTTGCTGATTGAAATCgg                                                                                                             <  1:193146/190‑1 (MQ=255)                                                                                                                                            gNNATCCCGNNAAGCTGGCTGGCAACAAAGTAATTACTCTTCACTTATTCCCCTCCGGTTCGCCGGAGGGTTTTTGGAGTTTGCTATGCGCGTATTGAt                                                                                                                                                                            <  2:19625/99‑1 (MQ=255)                                                                                                                                             cAATCCCGTTAAGCTGGCTGGCAACAAAGTAATTACTCTTCACTTATTCCCCTCCGGTTCGCCGGAGGGTTTTTGGAGTTTGCTATGCGCGTATTGATCAAAAACGGCACTGTCGTTAACGCAGATGGACAAGCCAAACAGGATTTGCTGATTGAAAGCGGGATTGTTCgn                                                                                                   <  1:77593/171‑2 (MQ=255)                                                                                                                                              aaTCCCGTTAAGCTGGCTGGCAACAAAGTAATTACTCTTCACTTATTCCCCGCCGGTNc                                                                                                                                                                                                                  <  1:199419/59‑1 (MQ=255)                                                                                                                                                         gctggctgGCAACAAAGTAATTACTCTTCACTTATTCCCCTCCGGTTCGCCGGAGGGTTTTTGGAGTTTGCTATGCGCGTATTGATCAAAAACGGCACTGTCGTTAACGCAGATAGACAAGCCAAACAGGATTTGCTGATTGAAAGCGGGATTGTTCGCCAGTTGGGCAACAATATTTCGCCGCAGCTCCCGTATGAAGaaa                                                        >  1:201450/1‑202 (MQ=255)                                                                                                                                                              ctggcAACAAAGTAATTACTCTTCACTTATTCCCCTCCGGTTCGCCGGAGGGTTTTTGGAGTTTGCTATGCGCGTATTGATCAAAAACGGCACTGTCGTTAACGCAGATGGACAAGCCAAACAGGATTTGCTGATTGAAAGCGGGATTGTTCGCCAGTTGGGCAACAATATTTCGCCGCAGCTCCCGTATGAAGAAATTGATGCAACTGGCTGTTACGTTTTCCCTGGCGGCGTGGATGTCCATACGCAtt  >  1:71046/1‑251 (MQ=255)                                                                                                                                                                       |                                                                                                                                                                                                                                                  CTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTCACTTATTCCCCTCCGGTTCGCCGGAGGGTTTTTGGAGTTTGCTATGCGCGTATTGATCAAAAACGGCACTGTCGTTAACGCAGATGGACAAGCCAAACAGGATTTGCTGATTGAAAGCGGGATTGTTCGCCAGTTGGGCAACAATATTTCGCCGCAGCTCCCGTATGAAGAAATTGATGCCACTGGCTGTTACGTTTTCCCTGGCGGCGTGGATGTCCATACGCATT  >  NC_000913/3009804‑3010211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑