Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,810,638	C→T	L99L (CTG→TTG)	ygaH →	L‑valine exporter, YgaH component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,638	0	C	T	100.0%	29.2 / NA	11	L99L (CTG→TTG)	ygaH	L‑valine exporter, YgaH component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

CCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAACACGCAGCATTATCATCCCAACACTGCTTAGTGCGCTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGC  >  NC_000913/2810400‑2810883                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      ccGCTATTTGCCGCTGTGCCTGCGTGTGGGTAATGCCCGCCCAACTAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCACCGCCTCGATATTCGCTCTGCTGGTTCTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAACACGCAGCATTATCATCCCAACACTGCTTAGTGCGTTGGCCTATGGGc                                                                                                                                                                                                                                           <  1:6281/251‑1 (MQ=255)                            gggTAATGCCCGCCCAACCACACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAACACGCAGCATTATCATCCCAACACTGCTTAGTGCGTTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATtatat                                                                                                                                                                                                                >  1:87809/1‑251 (MQ=255)                             ggTAATACCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAACACGCAGCATTATCATCCCAACACTGCTTAGTGCGTTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATtatat                                                                                                                                                                                                                >  1:56699/1‑250 (MQ=255)                                                                                                    cantctNCNNGNNGTCNCTACCGCACCAGAAGTGATGCNNGATACACGCNGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAANCACGCAGCANNATNATCCCNANNNTNNNTANTGCGTTGGCCTATGGGCTCNCCNNGAAAGTNATGGCGATTATATAACCCACAAGAAt                                                                                                                                                                                                   <  2:56699/189‑1 (MQ=255)                                                                                                                                                cacGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAACACGCAGCATTATCATCCCAACACTGCTTAGTGCGTTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACg                                                                                                  <  1:74317/245‑1 (MQ=255)                                                                                                                                                                        tCGGCTTCGCGGTACTGTGTGCCAGTTTCTATAAAACACGCAGCATTATCATCCCAACACTGCTTAGTGCGTTGGCCTATGGGCTCGCCTGGAAAGTGATggn                                                                                                                                                                                                                        <  1:54517/103‑2 (MQ=255)                                                                                                                                                                            cTTCGCGCTACTGGGTGCAAGTTTCTTTAAAACACGCAGCCTTATCATCCCAACCCTGCTTAGTGCGTTGGCCTATGGGCTCGCCTGGAAAGTGATGGc                                                                                                                                                                                                                        >  1:91103/1‑99 (MQ=255)                                                                                                                                                                                                              cGCAGCATTATCATCCCAACACTGCTTAGTGCGTGGGCTTATGGGCTCGTCTGGAAAGTGATGGCGATTATATACCCCACAAGAATCATTTTTCTAAAACATGACATTTCTTTTATTTGTCACTGGCGTTACTATATCGGCTGAAATTAATAAGGTCATACCGAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                                                <  1:192550/217‑1 (MQ=255)                                                                                                                                                                                                                nancaNTATCANCCCANCACTGCTTAGTGCGTNGGCCTATGGGCTCGCCTGGNNANTGATGNCGAtt                                                                                                                                                                                                                    <  2:110439/66‑1 (MQ=255)                                                                                                                                                                                                                           tcCCAACACTGATTAGTGCGTTGGCCTATAGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                                                    >  1:74350/1‑200 (MQ=255)                                                                                                                                                                                                                                          gTGCGTTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTACAACAATACATTTACTTTATTTGTCACTGTCGTTCCTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagc  >  1:35442/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      CCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACGATACACGCCGTTTCGTGCCCACGCTGGTCGGCTTCGCGGTACTGGGTGCCAGTTTCTATAAAACACGCAGCATTATCATCCCAACACTGCTTAGTGCGCTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGC  >  NC_000913/2810400‑2810883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑