Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	733,473	G→A	R1297R (CGG→CGA)	rhsC →	rhs element protein RhsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	733,473	0	G	A	100.0%	31.4 / NA	13	R1297R (CGG→CGA)	rhsC	rhs element protein RhsC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/6);  total (7/6)

GGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGTCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATTCACACCTATAAATTTAGG  >  NC_000913/733253‑733661                                                                                                                                                                                                                             |                                                                                                                                                                                             ngCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGCTATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGTCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGAGGAGAATCAAAAGGAGATGGTCTATggggg                                                                                                                                                                >  1:23348/2‑251 (MQ=255) ngCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGTCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGAGGAGAATCAAAAGGAGATGGTCTATggggg                                                                                                                                                                >  1:151569/2‑251 (MQ=255)                                                                                                                       tggaagaagAGGTCCAGATATATGGGGGAATCCGTTCTAGCAGGAATATCTTTGTGTCCCAGATGGTAAAGGTGTCTATACTTGTGGAGGGCAAGACCAACGAGGAGAATCAAAATGAGAGGGTCTATTGGGGCCATGTAAAGCAAGGAATTATACAATAGAAGCTTCGGGCGGTTGTGGCCTGGTTGAAACCGATAAGAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAag                                                         <  1:249404/233‑1 (MQ=255)                                                                                                                          aagaagCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGTCCCAGATGTTAAAGTGTACTATACTTTTGGTGGCCAAGACCAACGAGTAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAgc                                                                                                                                <  1:170785/162‑1 (MQ=255)                                                                                                                                  gTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGTCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGAGGAGCATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAATGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGtt                                                              >  1:249610/1‑220 (MQ=255)                                                                                                                                    ncAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGTCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGAGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAg                                                                                                                                                      >  1:51543/2‑130 (MQ=255)                                                                                                                                                      ccGTTCTATCATCAATATCTTTGTGTCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGAGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAATAATCTGCTGGGCTTTGTGACCTCGTTGAAAGCGATAATAGTTTTGTGGAGAAGTGGTTAGAAGGCAATGTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATTCACACCtat           <  1:240115/251‑1 (MQ=255)                                                                                                                                                               catcaATATCTTTGTGTCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGAGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAATCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATTCACACCTATAAATTTAgg  >  1:210235/1‑251 (MQ=255)                                                                                                                                                                          ttGTGGCCGAGATGGTAAAGGGGACTATACTTGTGGGGGCCAAGACCATCGAGGAGAATCAAAAGGAGATAGTCTATGGGGTCCAGGTAAAGCAAGTAATGATACAAAATAAGCTGCTGGcc                                                                                                                        <  1:248780/122‑1 (MQ=255)                                                                                                                                                                            gtgtCCCANATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACTAGGANNANNNANAGGAGATg                                                                                                                                                                           <  2:176768/69‑1 (MQ=255)                                                                                                                                                                            gtgtCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGAGGAGAATCAAAAGGCGATg                                                                                                                                                                           >  1:176768/1‑69 (MQ=255)                                                                                                                                                                                                    nnnnntgtnGTGGCCAAGACCAACGAGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGttt                                                                          <  2:151569/137‑1 (MQ=255)                                                                                                                                                                                                                 ccAAGACCAACGAGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTAt                                 >  1:75415/1‑170 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                             GGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGTCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATTCACACCTATAAATTTAGG  >  NC_000913/733253‑733661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑