Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,142,691	G→A	T100I (ACC→ATC)	hybC ←	hydrogenase 2 large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,142,691	0	G	A	100.0%	20.5 / NA	9	T100I (ACC→ATC)	hybC	hydrogenase 2 large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/4);  total (5/4)

AGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCG  >  NC_000913/3142476‑3142904                                                                                                                                                                                                                        |                                                                                                                                                                                                                      aGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTGTCAGCATGTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGATGTGCGCAGCCAGAATGATGTTACGGATGTATTgc                                                                                                                                                                                    <  1:197671/251‑1 (MQ=255)                                                                                cGGACTGTTCAGGTGCCAGGTCGAACCGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGCAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGATGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGc                                                                                                     >  1:188532/1‑251 (MQ=255)                                                                                      gTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGATGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGa                                                                                                                                                                 >  1:203293/1‑185 (MQ=255)                                                                                                                                                              gATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGATGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGAATCGCGGTTTTTcn                                                  <  1:76752/224‑2 (MQ=255)                                                                                                                                                                             cAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGATGTGCGCAGCCAGAATGATGTTACGGNTGTNTTGCNCGNTAACCGGAACGTCGANATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGNNGNNNTNCATACGcc                                                                                                    <  2:182649/159‑1 (MQ=255)                                                                                                                                                                                  ncGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGATGTGCGCAGCCAGAATGATGTTCCGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAgc                                                                                                                                                         >  1:30960/2‑101 (MQ=255)                                                                                                                                                                                   cGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGATGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTAGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGAATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCg  >  1:121421/1‑251 (MQ=255)                                                                                                                                                                                                 aattaactatatTGTCATGCGTGATGTGCGTAGCCAGAATGATGTTACGGATGTAGTGCGCGTTAACCGGAACGTCTATAGTCAGCACGCTTTCTGCCGCACGAGCGGAAGACAGCGCGTGAGTGGTAGTACATCCGCCACATATACGTTGCACAATCATCCAAGCGGCGCGCGAATCGCGGTGTTTCACGAt                                              <  1:228634/185‑1 (MQ=255)                                                                                                                                                                                                                cATGCGTGATGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGCTATTCAGCGCACTTTATGcc                                                                                                                                             >  1:105170/1‑83 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                      AGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCG  >  NC_000913/3142476‑3142904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑