Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,737	C→T	T141I (ACC→ATC)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,737	0	C	T	100.0%	27.6 / NA	12	T141I (ACC→ATC)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

ATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGC  >  NC_000913/3931516‑3931928                                                                                                                                                                                                                              |                                                                                                                                                                                                atatCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTTGAAATCGTCGCCCCGCAGCTGGATATCTGGATAGTTCCCCTCTCAATTATCGTtc                                                                                                                                                                    <  1:227906/251‑1 (MQ=255)                       gCAGATAACGCCGGTGCGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGGAATACGTCGGCGCGAACCACATAAATGCTGGTGATTATGGGGCTAGTCGGCGGCAGCTTTTTCTATGGTGAAGGCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATATCTGGATAGTTACCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTc                                                                                                                                               >  1:193946/1‑250 (MQ=255)                                                                         gCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGAGTATGGGGCTAATCGGCGGCAGCTGTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATATCTGGATAGTTCCCCTCTCAATTATc                                                                                                                                                                        <  1:126684/175‑1 (MQ=255)                                                                                     ggCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCCGCGGCAGCTTTTTCTATGCTAACGTCGTAATAACACCCGCTATTTCGGCGATGTCAGCCCTTGAAGGTCTGGAAATCGTCGCCCCGCCGCTGGATATCTGGATAGTTCCCCTCTCCATTATCGTTCTCACTTTATTATT‑ATGATTCAAAAACATGGCACGGCTATGGTCGGTCAGCTGTTTGCGCCGATCATGCTg                                                                                             >  1:166889/1‑237 (MQ=255)                                                                                        gcgAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCGGGAAATCGTCGCCCCGCAGCTGGATATCTGGATAGTTCCCCTCTCAATGATCTTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGTTATGGTCGGTAAGCTGTTTTCGCCGATCATGCTGACCTGGTTTTTGATTc                                                                             <  1:215829/251‑1 (MQ=255)                                                                                            aCCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCGATGGTGAAGTCGTCATAACGCCCGCGATTGCGGTGATGTCAGCCAGTGAAGGTCTGGAAATCGTCGCTCCTCAGCTGGATATCTGGATAGTTcc                                                                                                                                                                                     <  1:90582/143‑1 (MQ=255)                                                                                                                      gCTAATCGGCGGCAGCTTTTGCTATGGTGAAGGCGTCATAACACCCGCTTTTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGGCGCCCCGCAGCTGGATATCTGGATAGGTCCCCTCTCAATTATCGTTCTCACTTTAGTAGTTATGAGTCAAGAACATGGCGCGGCTATGGGTGGTATGCGGTTTGCGGTGATCATGCTGACCTGGTTCTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTg                                               <  1:125936/251‑1 (MQ=255)                                                                                                                              gcggcAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCCGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATATCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTTTTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTg                                                                                                           >  1:227437/1‑183 (MQ=255)                                                                                                                                    gCTTTTTCTATGGTGAAGTCGTCATANCACCCGNNATNTCGGTGATGTCAGCCATTGNAGNNNTNNNANTCGTANCCNNGCGGCTGGANATCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCANAAACATCGCNNGGCTATGGTc                                                                                                                        >  2:58654/1‑164 (MQ=255)                                                                                                                                    gCTTTTTCTATGGTGAAGTCGTCATACCACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCGGGAAATCGTCGCCCCGCAGCTGGATATCTGGATAGTTCCCCTCTCATTTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTc                                                                                                                        <  1:58654/164‑1 (MQ=255)                                                                                                                                                                  ccGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATATCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTgggg                                                               >  1:99054/1‑191 (MQ=255)                                                                                                                                                                    gCTATTTCGGTGATGTCAGACATTGCAGGTCTGGAAATCGTCGCCCCGCAGCTGGATATCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTACGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGc  >  1:193800/1‑250 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                ATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGC  >  NC_000913/3931516‑3931928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑