Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	126,388	G→A	V232I (GTT→ATT)	aceF →	pyruvate dehydrogenase, E2 subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	126,388	0	G	A	100.0%	12.9 / NA	7	V232I (GTT→ATT)	aceF	pyruvate dehydrogenase, E2 subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/0);  total (7/0)

GTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCG‑ACAAAGCTTCTATGGAAGTTCCGGCGCCG‑TTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGTTGAAGGCGCAGCGCCTGCGGCAGCTCCTGCGAAACAGGAAGCGGCAGCGCCGGCACCGGCAGCAAAAGCTGAAGCCCCGGCAGCAGCACCAGCTGCG  >  NC_000913/126236‑126630                                                                                                                                                         |                                                                                                                                                                                                                                                     gTGAAGCAGGCGCGGCAACTCCGGCCGCTACACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGACAGACGTTAACGTTCCGGATATCGGCGGTGACGAAGGTGGAGTGAATGAAGTGATGGTGGAAGAGGGGGGCAAAATTGCCGCCGAAAAGACCCTGAACACCGTAGAAGACGCACAAA‑CTTCTATGGAAATTCCGGCGCCGGTTTGC‑GGCGTCGTGGAGGAACTGAAAGGCaa                                                                                                                                                   >  1:100215/1‑250 (MQ=255)                      cntccGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGTCGAAGGTGAAGTGACTGAAGTGATGGGGAAAGTGGGCGACCAAATTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCG‑ACCAAAATTCTATGGAAGTTCCGGCGCCC‑ttttccg                                                                                                                                                                            >  1:199755/4‑200 (MQ=255)                       ggCCGCTAAACAGGAAGCAGATCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTCACGTTCCGGATATCGGCGGTGGCCAAGTTGGAATGACTGGAGTGATGGGGAAAGTGGGCGGCACAATTTCCGCTGAACa                                                                                                                                                                                                                                         >  1:89525/1‑144 (MQ=255)                                                                                                        cggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAATTGCCGCTGAACAGTCACTGATCACCGTAGa                                                                                                                                                                                                                       >  1:60615/1‑81 (MQ=255)                                                                                                              ngAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAATTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCG‑ACAAAGCTTCTATGGAAGTTCCGGCGCCG‑TTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGTTGAAGGCGCAGCGCCTGCGGCAGCTCCTGCGAAACAGGAAGCGGCAGCGccggcaccggc                                       >  1:152032/2‑249 (MQ=255)                                                                                                                  gTTGAAGTGACTGACGTGATGGTGAAAGTGGGCGACAAAATTGCCTCTGAACAGTCACTGATCACCGTAGAAGGCA‑ACAAAGCTTCTATGGAAGTTCCGGCGCCG‑TTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGCAAACTGGCACGCTg                                                                                                                    >  1:71958/1‑168 (MQ=255)                                                                                                                                                  cGACAAAATTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCG‑ACAAACCTTCTATGGAAGTTCCGGCGCCG‑TTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAACGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGTTGAAGGCGCAGCGCCTGCGGCAGCTCCTGCGAAACAGGAAGCGGCAGCGCCGGCACCGGCAGCAAAAGCTGAAGCCCCGGCAGCAGCACCAGCTGCg  >  1:138212/1‑250 (MQ=255)                                                                                                                                                         |                                                                                                                                                                                                                                                     GTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCG‑ACAAAGCTTCTATGGAAGTTCCGGCGCCG‑TTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGTTGAAGGCGCAGCGCCTGCGGCAGCTCCTGCGAAACAGGAAGCGGCAGCGCCGGCACCGGCAGCAAAAGCTGAAGCCCCGGCAGCAGCACCAGCTGCG  >  NC_000913/126236‑126630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑