Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	812,585	C→T	L106F (CTT→TTT)	bioD →	dethiobiotin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	812,585	0	C	T	100.0%	16.1 / NA	9	L106F (CTT→TTT)	bioD	dethiobiotin synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/6);  total (3/6)

CAAAGGCAGCAGGCTACCGGACGGCAGGTTATAAACCGGTCGCCTCTGGCAGCGAAAAGACCCCGGAAGGTTTACGCAATAGCGACGCGCTGGCGTTACAGCGCAACAGCAGCCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGG  >  NC_000913/812349‑812792                                                                                                                                                                                                                                             |                                                                                                                                                                                                                cAAAGGCAGCAGGCTACCGGACGGCAGGTTATAAACCGGTCGCCTCTGGCAGCGAAAAGACCCCGGAAGGTTTACGCAATAGCGACGCGCTGGCGTTACAGCGCAACAGCAGCCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAGCCCTCTGCGCCGCACATCATCAGCGCGCAATAGGGCAGACCGATAGAATCAGTGGTAATGAGCGCCGGATTACGCGCGTTTGAACAACAGGCt                                                                                                                                                                                                   <  1:124340/251‑1 (MQ=255)                         cAGGTTATAAACCGGTCGCCTCTGGCAGCGAAAAGACCCCGGAAGGTTTACGCAATAGCGACGCGATGGCGTCACAGCGCAACAGCAGCCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCAACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGTTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCt                                                                                                                                                                           >  1:231425/1‑251 (MQ=255)                                       gTCGCCTCTGGCAGCGAAAAGACCCCGGAAGGTTTACGCAATAGCGACGCGCTGGCGTTACAGCGCAACAGCAGCCTGCAGCTTGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCATCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACTCGCGTTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTAc                                                                                                                                                             <  1:166389/251‑1 (MQ=255)                                                            cccccGGAAGGTTTACGCAATAGCGACGCGTTGGCGTTACAGCGCAACAGCAGCCTTCAGCTTGATTACGCAACAGTAAATCCTTACACCTGCGCAGAACCCTCTTCTCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGTTTGAACAACAGGCTGACGGGGTGTTAGTGGAATGTGCTGGCGGCTGGTTTACGCCGCTTTCTGAcactttcac                                                                                                                                        <  1:182751/250‑1 (MQ=255)                                                                           cGCAATAGCGACGAGCTGGCGTTACAGCGCAACAGCAGCCTGCAGCTGGATAACGACCCAGTAAATCCTTACAACTTCGCAGAACCCACTTCGCCGCACATCATCAGCGAGCAAGAGGGCAGACCGATAGAATCATTGGTACTGAGCGTAGGATTACGCGCGTTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGATTACGCAGCTTTCTGACACTTTCACTTTTGCAGATTGGGt                                                                                                                         >  1:242754/1‑251 (MQ=255)                                                                                               gTTACAGCGCAACAGCAGCCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACGTCGCCGCACATCATCAGCGCGCAACAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGTTTg                                                                                                                                                                                                              <  1:242675/146‑1 (MQ=255)                                                                                                            agcagcCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGTTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTg                                                                                             <  1:58697/246‑1 (MQ=255)                                                                                                                                                    ccTTCGCAGAACACACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGCCCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGTTTGAAAAACAGCCTGACTGGGCGCTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTCTCTGAcactttcac                                                                                                                                        >  1:249262/1‑163 (MQ=255)                                                                                                                                                                                                  cAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGTTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTGCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACGCTGGCGGGTTGGGTgg  <  1:83162/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                CAAAGGCAGCAGGCTACCGGACGGCAGGTTATAAACCGGTCGCCTCTGGCAGCGAAAAGACCCCGGAAGGTTTACGCAATAGCGACGCGCTGGCGTTACAGCGCAACAGCAGCCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGG  >  NC_000913/812349‑812792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑