Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,093	G→A	noncoding (73/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,093	0	G	A	100.0%	25.8 / NA	13	noncoding (73/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/3);  total (10/3)

CCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGT  >  NC_000913/4605846‑4606311                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                            ccGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCCGATGGGATTAGTCTTGACGCCGATGATGCTGTATAACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCACGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGAGAc                                                                                                                                                                                                                          >  1:181287/1‑251 (MQ=255)                                       aTCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGAAAAGATAATTGGTGCGAGGGGGGAGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACATGaa                                                                                                                                                                                    >  1:6839/1‑250 (MQ=255)                                                     ggCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGAGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                                                                                                     <  1:73987/251‑1 (MQ=255)                                                                                     ncGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGAGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACtt                                                                                                                                     >  1:152211/2‑251 (MQ=255)                                                                                                                     gCATTACATTTTGCTCCCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGATGCGAGGGGGGAGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCCTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg                                                                                                     >  1:86417/1‑251 (MQ=255)                                                                                                                     gCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGAGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                                                                                                                     >  1:166913/1‑203 (MQ=255)                                                                                                                                                           cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGAGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCtt                                                                                                                                         >  1:37377/1‑177 (MQ=255)                                                                                                                                                                                                  gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGAGCCTTGACACCCCC‑CGTCCGTAAGGac                                                                                                                                                                                                 >  1:44740/1‑82 (MQ=255)                                                                                                                                                                                                         cAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGAGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGa                                                                          >  1:90685/1‑194 (MQ=255)                                                                                                                                                                                                         cAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGAGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                       >  1:14742/1‑245 (MQ=255)                                                                                                                                                                                                              nnnnnnnnnnaanNNNNGTANAGATAATTGGTGCGAGGGGGGAGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                             <  2:152211/144‑1 (MQ=21)                                                                                                                                                                                                                 aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGAGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAACTGATGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc          >  1:12156/1‑250 (MQ=255)                                                                                                                                                                                                                        aaaGGAAGGAAAGATAATTGGTGCGAGGGGGGAGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCTCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt  <  1:229679/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                            CCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGATGATGCTGTATCACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGA‑ACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGT  >  NC_000913/4605846‑4606311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑