Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 400,626 C→T D100N (GAT→AAT)  ddlA ← D‑alanine‑‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913400,6260CT93.3% 31.4 / NA 15D100N (GAT→AAT) ddlAD‑alanine‑‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (8/6);  minor base G (0/1);  total (8/7)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGC  >  NC_000913/400385‑400861
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           
aaCCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATTCACCGTCgg                                                                                                                                                                                                                                    <  1:38020/251‑1 (MQ=255)
                                                                                                            gTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACAATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATTCACCGTCGGc                                                                                                                                                                                                                                   >  1:108748/1‑144 (MQ=255)
                                                                                                                naTCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATTCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCAttt                                                                                                                    >  1:66043/2‑251 (MQ=255)
                                                                                                                          cATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGCCAATCGGGAAAATGACATTCAACGTCGGCAACGGCTg                                                                                                                                                                                                                           >  1:39994/1‑138 (MQ=255)
                                                                                                                             aCAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATTCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGc                                                                                                       <  1:1422/251‑1 (MQ=255)
                                                                                                                                     ctgAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATGCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaacn                                                                                                                                              <  1:28696/204‑2 (MQ=255)
                                                                                                                                                                   gTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGTGTACTGTGGACCATAGGGCACATGACATTCACCGTCGGCAACGGCTGACCGTTTTGAGCGTCGATAAGCTGCTGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCATTGTTTacgccg                                                                  >  1:231118/1‑245 (MQ=255)
                                                                                                                                                                           gCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATTCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCANCTg                                                                                                                                                                           >  2:150914/1‑137 (MQ=255)
                                                                                                                                                                           gCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATTCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCGGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGtn                                                                                       <  1:150914/221‑2 (MQ=255)
                                                                                                                                                                                          cncTNCAAGGNACCANCTTCGCCCAGCGTACNGTGGACAAACGGGAAAATGNNANTCACCNTCGGc                                                                                                                                                                                                                                   <  2:108748/66‑1 (MQ=255)
                                                                                                                                                                                                           ttCGCCCAGCGTACCGAGGACAATCGGGAAAATGACATTCAACGTCGGCAACAGCAAACACATTCGCGCGTCGATCAACTGCTGATCATGTTTACCTGGCACCTGCGCCAGGCTGGACGCCGAAGGGAGCAACGCAATATGGGAAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCCGCAGCACAACGTCGAAGCGACTTTTATCAATGGCAt                         >  1:143570/1‑251 (MQ=255)
                                                                                                                                                                                                               cccAGCGTACCGTGGACAATCGGGAAAATGACATTCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAc                     >  1:97822/1‑251 (MQ=255)
                                                                                                                                                                                                                               cAATCGGGAAAATGACATTCACCGGCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCa                                                                                                                                                                               <  1:139150/81‑1 (MQ=255)
                                                                                                                                                                                                                                    gggAAAATGACATTCACCGTCGGCAACGGCTGACCGTGTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGc                                                                                      <  1:44109/165‑1 (MQ=255)
                                                                                                                                                                                                                                     ggAAAATGACATTCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGc  >  1:2892/1‑248 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           
AACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGC  >  NC_000913/400385‑400861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: