Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,439,389	G→A	P41S (CCG→TCG)	yhdN ←	DUF1992 domain‑containing protein YhdN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,439,389	0	G	A	100.0%	26.7 / NA	14	P41S (CCG→TCG)	yhdN	DUF1992 domain‑containing protein YhdN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/5);  total (9/5)

GACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTT  >  NC_000913/3439245‑3439601                                                                                                                                                 |                                                                                                                                                                                                                     gACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGATGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTgg                                                                                                            >  1:184937/1‑251 (MQ=255)                         gATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCAGTCTTCAGCAAGCGATACCCCGCACGTAATTCCGATGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTc                                                                                                                                     <  1:141851/202‑1 (MQ=255)                                                                gAATTGCTTCTCTCCGTTGCTCCAGTTCTGGCGGTAAGCACCCGGCATTCCTCCGCAAGCGATATTCCGAACGTCATTCCGATGGCACGCGAGAATCCTCATCCCGTATCAATGGTTAGCCGCTACCTGCCAGGTTATCAAACTCCCCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACcac                                                                                                >  1:121697/1‑200 (MQ=255)                                                                                     cAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGATGGCACGAGAGAATCATCATCCAGAATCCATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTTTGCTATATGgcgc                                                                                                                        >  1:133130/1‑155 (MQ=255)                                                                                           cTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGATGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACccg                  >  1:4879/1‑251 (MQ=255)                                                                                           cTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGAGACCCCGCACGTAATTCCGATGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTg                                                                                                                                                                       <  1:127951/102‑1 (MQ=255)                                                                                             ggCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGATGGCAAGGGGGAATCATCATCCAGTATCAATGGTTCGCCGCTACTTGCCAGGGTATCCAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTg                                                                                                                    >  1:132171/1‑151 (MQ=255)                                                                                               nnnnnaGCNACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGATGGCACGTGGGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATgg                                                                                                                           <  2:151465/137‑1 (MQ=255)                                                                                               nggTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGATGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATgg                                                                                                                           >  1:151465/2‑142 (MQ=255)                                                                                                ggTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGAACGTAATTCCGATGGCAAGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTATGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATaaaaaaa                      >  1:188841/1‑242 (MQ=255)                                                                                                    aGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGATGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACATGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCggg             >  1:223909/1‑247 (MQ=255)                                                                                                           ggCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGATGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttttt  >  1:12602/1‑251 (MQ=255)                                                                                                                    cAGCAAGCGATACCCCGCACGTAATTCCGATGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGc                                                                                                                                  <  1:129530/114‑1 (MQ=255)                                                                                                                     aGCAAGCGATACCCCGCACGTAATTCCGATGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACGCACCATTCGCTTTCGCATCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTAGTGTAGATAAGTgg                                            <  1:80673/199‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                                                                                     GACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTT  >  NC_000913/3439245‑3439601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑