Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,608,550	C→T	R158H (CGT→CAT)	yneE ←	PF01062 family inner membrane protein YneE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,608,550	0	C	T	100.0%	35.0 / NA	13	R158H (CGT→CAT)	yneE	PF01062 family inner membrane protein YneE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/6);  total (7/6)

GAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGC  >  NC_000913/1608404‑1608777                                                                                                                                                   |                                                                                                                                                                                                                                    gAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATATGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCa                                                                                                                                                                      >  1:78774/1‑210 (MQ=255)       gCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATATGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATc                                                                                                                                                                                             <  1:229483/181‑1 (MQ=255)                    gcgcTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAACGCTGTCCATTGCGGCGCTGAACCGCCAACTATTCTCCCATTATTAACAAGATATGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTACTGTCATGCGTAAACAGTGGGCGAAAGCGAttt                                                                                                          >  1:222281/1‑251 (MQ=255)                     cgcTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCTATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCAGTGCGGCGCTGAACCGCCAACCATTCGCCCATTATTAACAAGATATGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCTCTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTg                                                                                                         <  1:191726/251‑1 (MQ=255)                               cTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATATGGTTAGCTGGAGAGTTCGCAGCCAGTACACGCTGAAGATCTTCAGTCTTga                                                                                                                                                                                  >  1:96931/1‑168 (MQ=255)                                     ggACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATATGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTcc                                                                                         <  1:4698/251‑1 (MQ=255)                                              aaaTATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATATGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTac                                                                                    >  1:93560/1‑247 (MQ=255)                                                                                                   ccATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATATGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAat                           >  1:194560/1‑251 (MQ=255)                                                                                                           gcgcTGAACCGCCAACCATTCTCCCATTATTAACAAGATATGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGttttt                                                        <  1:21342/214‑1 (MQ=255)                                                                                                           gcgcTGAACCGCCAACCATTCTCCCATTATTAACAAGATATGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCNNGAGATan                                                                                                                                                                             >  2:21342/1‑96 (MQ=255)                                                                                                                     gCCAACCATTCTCCCATTATTAACAAGATATGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTg                                                                                                                                               >  1:13726/1‑117 (MQ=255)                                                                                                                            aTTCTCCCATTATTAACAAGATATGTTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGACTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAAGGGCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTcgc  <  1:245660/251‑1 (MQ=255)                                                                                                                                       annaACAAGNNATGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTg                                                                                                                                               <  2:13726/99‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                                                                                                    GAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGC  >  NC_000913/1608404‑1608777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑