Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,599,597	G→A	P205S (CCT→TCT)	lsrK ←	autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,599,597	0	G	A	100.0%	30.9 / NA	13	P205S (CCT→TCT)	lsrK	autoinducer‑2 kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/5);  total (8/5)

TTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGGCCAGCCATATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATGGTGATGGTTGATGCCTGACGGTAAATATCGGAACGATGGTGCGCCAGCCAAAGTAATCTGGGGATGGCACTTAAAGCCAGTGTTTGTCCGGTCGCGCGATAAACTTCGTTTTCAAAGGTATTGTTGTGC  >  NC_000913/1599406‑1599832                                                                                                                                                                                                |                                                                                                                                                                                                                                            ttGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCTAACGACCACCGGAGTGCCCTCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCGCTCCTAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATAGCGGCACGTAGGCCAGCCATATCCAGCAATGCAGATGTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATcc                                                                                                                                                                                   <  1:116583/250‑1 (MQ=255)            gCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCGTCCTCCAACGACCACAGGAGTGCCCGCCTTCAGACCGCAGCGTTACGCAGCTTGTGAACTTACCACGCCCAGCAATGCGCCGGTTTCTTTGCCAGGAGAAAGAATATCGGCACGTAGGCCAGCCATATCCAGCAATGCAGATTTCCAGTCACGGGTGGTTAGATCAAGAAGGCACGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTcg                                                                                                                                                                       >  1:249522/1‑251 (MQ=255)                                       gCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGACCTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGGCCAGCCATATCCAGCAATGCAGATTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTcgccg                                                                                                                                                                    >  1:103302/1‑227 (MQ=255)                                          gCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGGCCAGCCATATCCAGCAATGCAGATTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTg                                                                                                                                         >  1:42269/1‑251 (MQ=255)                                                 ncctcctCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTTCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGGCCAGCCATATCCAGCAATGCAGATTTCCAGTCACGGGTGGTTAGATCa                                                                                                                                                                                                                    >  1:68248/2‑169 (MQ=255)                                                                              ccTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGGCCAGCCATATCCAGCAATGCAGATTTCCAGTCACGGGGGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATGGTGATGGTTGATGCCTGACGGTAAATATCg                                                                                                     >  1:14683/1‑251 (MQ=255)                                                                                             gTTCCGCCGCTTGTGAACTTACCACGNCCAGCANNGTNCCGGTTTCTTTGACAGGAGNAANNNTNNNGNCACGTNGGNNAGCCATATCNAGCAATGCAGATTTCCAGTCACggg                                                                                                                                                                                                                               >  2:61490/1‑114 (MQ=255)                                                                                             gTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGGCCAGCCATATCCAGCAATGCAGATTTCCAGGCACggg                                                                                                                                                                                                                               <  1:61490/114‑1 (MQ=255)                                                                                                                                             ngACAGGAGAAAGAATATCGGCACGTAGGCCAGCCATATCCAGCAATGCAGATTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGGCGCTGATCATGGTGATGGTTGATGCCTGACGGTAAATATCGGAACGATGGTGCGCCAGCCAAAGTAATCTGGGGATGGCACTTAAAGCCAGTGTTTGTCCg                                         >  1:157459/2‑248 (MQ=255)                                                                                                                                                               cGGCACGTAGGCCAGCCATATCCAGCAATGCAGATTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATGGTGATGGTTGATGCCTGTCGGTAAATATCGGAACGATGGTGCGCCAGCCAAAGTAATCTGGGGATGGCACTTAAAGCCAGTGTTTGTCCGGTCGCGCGATAAACTTCGtt                     <  1:134745/250‑1 (MQ=255)                                                                                                                                                                  cACGTAGGCCAGCCATATCCAGCAATGCAGATTTCCANTCACGNGNGNNTANNTCaagaag                                                                                                                                                                                                               <  2:166304/61‑1 (MQ=255)                                                                                                                                                                  cACGTAGGCCAGCCATATCCAGCAATGCAGATTTCCAGTCACGGGTGGTTAGATCaagaag                                                                                                                                                                                                               >  1:166304/1‑61 (MQ=255)                                                                                                                                                                                  atCCAGCAATGCAGATTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATGGTGATGGTTGATGCCTGACGGTAAATATCGGAACGATGGTGCGCCAGCCAAAGTAATCTGGGGATGGCACTTAAAGCCAGTGTTTGTCCGGTCGCGCGATAAACTTCGTTTTCAAAGGTATTGTtgtgn  <  1:78022/250‑2 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                                                                            TTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTTGTGAACTTACCACGCCCAGCAATGTGCCGGTTTCTTTGACAGGAGAAAGAATATCGGCACGTAGGCCAGCCATATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGATCATGGTGATGGTTGATGCCTGACGGTAAATATCGGAACGATGGTGCGCCAGCCAAAGTAATCTGGGGATGGCACTTAAAGCCAGTGTTTGTCCGGTCGCGCGATAAACTTCGTTTTCAAAGGTATTGTTGTGC  >  NC_000913/1599406‑1599832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑