Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,347,028	C→T	G100D (GGC→GAC)	fumB ←	fumarase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,347,028	0	C	T	90.9%	20.3 / ‑6.3	11	G100D (GGC→GAC)	fumB	fumarase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (6/4);  minor base A (1/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCC  >  NC_000913/4346842‑4347201                                                                                                                                                                                           |                                                                                                                                                                              gTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGAGCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGTCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGc                                                                                                               <  1:84034/251‑1 (MQ=255)                                            gtgAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGTCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTGCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTGTGcc                                                                    <  1:90921/251‑1 (MQ=255)                                                                        ggTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGTCTTTGGCGGCGATTTCGGCGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGa                                         >  1:125419/1‑250 (MQ=255)                                                                                     ccTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGTCTTTGGCGGCGATTTCGAAGTTTCTTAAGAATTGCAGCGCCACTTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCGGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGAAAGGCTTGCTgcgc                           <  1:186606/251‑1 (MQ=255)                                                                                                 gTTTCTTCATCACCGCCGCCGGTCCACAAGCGCTGGCCCTTTTTACCGGCCATGATCCCGCTCCCGGTCTCCTGGCAGGGCGCCACCACCACCTTGGCGGCAATTTCGGAGTTTCTTAAGAAATGCAGCGCCACGTACTTATCGTTTTCCCTGGCTTCTGGATCGTGACGAATAGCCGCAACCTCTTTCCGGTGTGCCGGCCGGAGCATAAAACAAGCGGCGTGAAAGACCTCCTGCTCCAGCCGGGTc                 >  1:122802/1‑249 (MQ=255)                                                                                                        catcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGAGCCGGTATCCTGGCAGGTCGGCAGCACGTATTTGGCGCCGATTTAGGAGTTTCTTCAGAATTGAAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGCAGAATAGCCGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTAAGGGCTTCt        >  1:182478/1‑251 (MQ=255)                                                                                                           caCCGCCGCCGGTCCACACGCGCTGGCATTTTTTACCGACGATGATCGCGGTGACGGTATCCTCGAAAATCGGCAGCACGTCTTTGGCGGCGATTTCGGAGTTTCTTAAGACTTGCAGCGCCACGTACTTGACGTTTTCGCCGCCTTCTGGATCGTGAAGAATAGCTGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATCAAAGAAGCGTCGTGAAAGGCTTGCTGCGCAAGCAGGGTCAGGGCTTCTGGt     >  1:138278/1‑251 (MQ=255)                                                                                                                                   ggCCTTTTTTCCCGACGATGATCGCGGTTCCGGTATCCTGGCAGGTCGGCAGCACGTCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGAACTTGTCGTTTTCGCGGGCTTCTTGATCGTGAAGAATAGGCGCAACCTGGTTCTTGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTCAAATGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTcc  <  1:132026/230‑1 (MQ=255)                                                                                                                                              ncgacgaTGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGTCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGACGAATAGCCGCAACCTGTTTCTGGTGTGCCggg                                                                 >  1:52148/2‑156 (MQ=255)                                                                                                                                                   gatgatCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGTCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGAGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGAAAGGc                                    >  1:104596/1‑180 (MQ=255)                                                                                                                                                         cgcgGTGCCGGTATCCTGGCAGGTCGGCAGCACGTCTTTGGCGGCGATTTCGGTGTTTCTTAAGTATTGCAGCGCCACGTAc                                                                                                                                >  1:116626/1‑82 (MQ=255)                                                                                                                                                                                           |                                                                                                                                                                              GTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCC  >  NC_000913/4346842‑4347201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑