Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	158,903	C→T	G75D (GGC→GAC)	pcnB ←	poly(A) polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	158,903	0	C	T	100.0%	32.3 / NA	14	G75D (GGC→GAC)	pcnB	poly(A) polymerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/6);  total (8/6)

GAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGCCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTC  >  NC_000913/158669‑159145                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   gAGCCGAAAATGGTGTCGCGCAGCAACATGCCGTTTGGCCCGCGTTGGGAGGTCGTGCGGTCACTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATGATCCAGACGGAAACGGCGACCCCCCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACacgatcgg                                                                                                                                                                                                                                    >  1:22844/1‑244 (MQ=255)                   cgcAGCAACTTGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGCTAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCTTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAAccan                                                                                                                                                                                                                  <  1:77109/251‑2 (MQ=255)                                 gTTTTGCCCGCGTTGGGAGGTCGTGAGGTCGCTGACGGTAGCTGCGTGGTGTGAAAGGAAGGTGGGAACTGCGATGATCTACGGGGCACGCATGACATGAGCCAGACGTGAAGGGCAAGCCACCAGGCGGCAGATCCGGAGCAGTGTGAGCACCTGCGCAGACGAGGAGGTCGTGGTTACGTCAAAATCTTTCGGCTTTTTGTAAAGTACCGGGTCGCGCACGCCGCAGCCAACCAACCAGGCTTCGAATc                                                                                                                                                                                                    >  1:132415/1‑251 (MQ=255)                                   tttGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTGGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCATGCTTCGTATcc                                                                                                                                                                                                   <  1:92005/250‑1 (MQ=255)                                                                                                                                      ccAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACgg                                                                                               >  1:12812/1‑251 (MQ=255)                                                                                                                                             gAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCTCCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTGCCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCtgtg                                                                                                    <  1:138367/239‑1 (MQ=255)                                                                                                                                               aaCGGCGACCCACCAGGCGGCANTTACGGAACNNTTNGCGCANCNNNTNNNGCNTGGCGTTAGTGGTTACGTCNAANNCTTTCGNCTTTTTGTCAAGTAACAGGTCGCGCa                                                                                                                                                                                                                                  <  2:57247/111‑1 (MQ=255)                                                                                                                                               aaCGGAGACCCACCAGGCGGCCGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCa                                                                                                                                                                                                                                  >  1:57247/1‑111 (MQ=255)                                                                                                                                                  gnngccccANNAGGCGGCAGTTACGCAACAGTTTGCGCCCCTGCTATGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACa                                                                                                                                                                                                                                           <  2:22844/94‑1 (MQ=255)                                                                                                                                                       nccaccaGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACgg                                                                                               >  1:154211/2‑234 (MQ=255)                                                                                                                                                                     gTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTCGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCATATCCCGATTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTc                                                                                 >  1:189324/1‑234 (MQ=255)                                                                                                                                                                       tactgAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGGGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTTTCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAg                                                                                                                                     <  1:162046/176‑1 (MQ=255)                                                                                                                                                                              cAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGc                                                        >  1:162792/1‑250 (MQ=255)                                                                                                                                                                                                                                   ncTTTTTGTCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTc  >  1:64865/2‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   GAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATGAGCCAGACGGAAACGGCGACCCACCAGGCGGCAGTTACGGAACAGTTTGCGCACCTGCTCAGGCGTGGCGTTAGTGGTTACGTCAAAATCTTTCGGCTTTTTGCCAAGTAACAGGTCGCGCACGCCGCCGCCAACCAGCCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTC  >  NC_000913/158669‑159145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑