Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,537,058	G→A	G83S (GGC→AGC)	greB →	transcription elongation factor GreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,537,058	0	G	A	100.0%	23.0 / NA	9	G83S (GGC→AGC)	greB	transcription elongation factor GreB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/6);  total (3/6)

CCCTGGTTACCCGGGAAGGGTATGAAAAACTCAAACAAGAGCTTAATTATCTCTGGCGTGAAGAACGCCCGGAGGTCACAAAAAAGGTGACCTGGGCCGCAAGTCTGGGCGACCGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGA  >  NC_000913/3536822‑3537280                                                                                                                                                                                                                                             |                                                                                                                                                                                                                               cccTGGTTACCCGGGAAGGGTATGAAACACTAAAACAAGAGCTTAATTATCTCTGGCGTGAAGAACGCCCGGAGGTCACAAAAAAGGTGACCTGGGCCGCAAGTCTGGGAGACAGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAAGCAAAGTCtttttt                                                                                                                                                                                                                  >  1:84767/1‑251 (MQ=255)     ggTTACCCGGGAAGGGTATGAAAAACTCAAACAAGAGCTTAATTATCTCTGGTGTGAAGAACGCCCGGAGGTCACAAAAAAGGTGACCTGGGCCGCAAGTCTGGGCGACCGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGGCGATTACTCCCCTCAGCAGGAAAGCAAAGTCTTTTTTgg                                                                                                                                                                                                                <  1:134923/249‑1 (MQ=255)                                                                                              gggCCGCAAGTCTGGGCGACCGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACGCCCCTCAGCAGGAAAGCAAAGTCTTTTTTgg                                                                                                                                                                                                                <  1:203151/160‑1 (MQ=255)                                                                                                 ccGCAAGTCTGGGCGACCGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAAGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATctct                                                                                                                  >  1:124336/1‑251 (MQ=255)                                                                                                          tGGGCGACCGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAAGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCAcn                                                                                                                                                                       <  1:204584/189‑2 (MQ=255)                                                                                                                                                            cGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAAGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCggtggn                                                       <  1:203797/251‑2 (MQ=255)                                                                                                                                                                                          aCTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAAGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGCGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGAGAGCTGGt                          >  1:15495/1‑250 (MQ=255)                                                                                                                                                                                                            ctcAAAATCGTCGATTACTCCCCTCAGCAGGAAAGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGAGTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCtn                                                               <  1:26985/195‑2 (MQ=255)                                                                                                                                                                                                                 aaTCGTCGATTACTCCCCTCAGCAGGAAAGCAAATTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCTATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGTTGAAGTCCCCTGTCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGa  <  1:122777/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                               CCCTGGTTACCCGGGAAGGGTATGAAAAACTCAAACAAGAGCTTAATTATCTCTGGCGTGAAGAACGCCCGGAGGTCACAAAAAAGGTGACCTGGGCCGCAAGTCTGGGCGACCGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGCGTCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGA  >  NC_000913/3536822‑3537280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑